Evidence map›Paper›PMID 36824749›Full record

ArticlebioRxiv : the preprint server for biology2023

A single-cell map of antisense oligonucleotide activity in the brain.

Meredith A Mortberg, Juliana E Gentile, Naeem Nadaf, Charles Vanderburg, Sean Simmons, Dan Dubinsky, Adam Slamin, Salome Maldonado, Caroline L Petersen, Nichole Jones and 4 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Meredith A MortbergStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Juliana E GentileStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Naeem NadafStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Charles VanderburgStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Sean SimmonsStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Dan DubinskyGenomics Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.
Adam SlaminGenomics Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.
Salome MaldonadoGenomics Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.
Caroline L PetersenGenomics Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.
Nichole JonesGenomics Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.
Holly B KordasiewiczIonis Pharmaceuticals, Carlsbad, CA, 92010, USA.
Hien T ZhaoIonis Pharmaceuticals, Carlsbad, CA, 92010, USA.
Sonia M VallabhStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Eric Vallabh MinikelStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard,Cambridge, MA, 02142, USA.
Broad Institute · USIonis Pharmaceuticals (United States) · US

Funding

How Substrate Dosage Drives Prion Disease KineticsR01NS125255 · NINDS · BROAD INSTITUTE, INC. · PI Sonia Minikel Vallabh · 2022 to 2026
$2.7M
Biomarkers to enable ASO prevention trials in genetic prion disease carriersR21TR003040 · NCATS · MASSACHUSETTS GENERAL HOSPITAL · PI ARNOLD, STEVEN E · 2019 to 2020
$485k
NCATS NIH HHS R21 TR003040NINDS NIH HHS R01 NS125255
6 · The paper itself

Abstract

Antisense oligonucleotides (ASOs) dosed into cerebrospinal fluid (CSF) distribute broadly throughout the brain and hold the promise of treating myriad brain diseases by modulating RNA. CNS tissue is not routinely biopsied in living individuals, leading to reliance on CSF biomarkers to inform on drug target engagement. Animal models can link CSF biomarkers to brain parenchyma, but our understanding of how individual cells contribute to bulk tissue signal is limited. Here we employed single nucleus transcriptomics on tissue from mice treated with RNase H1 ASOs against

Identifiers

PMID36824749
PMCPMC9948956
OpenAlexW4320715457

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.