Evidence map›Paper›PMID 36824746›Full record

ArticlebioRxiv : the preprint server for biology2023

The Energetics and Ion Coupling of Cholesterol Transport Through Patched1.

T Bertie Ansell, Robin A Corey, Lucrezia Vittoria Viti, Maia Kinnebrew, Rajat Rohatgi, Christian Siebold, Mark S P Sansom

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

T Bertie AnsellDepartment of Biochemistry, South Parks Road, Oxford, OX1 3QU, UK.
Robin A CoreyDepartment of Biochemistry, South Parks Road, Oxford, OX1 3QU, UK.
Lucrezia Vittoria VitiDivision of Structural Biology, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK.
Maia KinnebrewDepartments of Biochemistry and Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Rajat RohatgiDepartments of Biochemistry and Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Christian SieboldDivision of Structural Biology, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK.
Mark S P SansomDepartment of Biochemistry, South Parks Road, Oxford, OX1 3QU, UK.
University of Oxford · GBCentre for Human Genetics · GBStanford University · US

Funding

Supplement application for an Olympus automated microscopeR35GM118082 · NIGMS · STANFORD UNIVERSITY · PI RAJAT ROHATGI · 2016 to 2026
$7.6M
Signal Transduction by OxysterolsR01GM106078 · NIGMS · STANFORD UNIVERSITY · PI COVEY, DOUGLAS F, ROHATGI, RAJAT · 2014 to 2017
$1.6M
Biotechnology and Biological Sciences Research Council BB/R00126X/1Cancer Research UK 26752NIGMS NIH HHS R01 GM106078NIGMS NIH HHS R35 GM118082Wellcome Trust
6 · The paper itself

Abstract

Patched1 (PTCH1) is the principal tumour suppressor protein of the mammalian Hedgehog (HH) signalling pathway, implicated in embryogenesis and tissue homeostasis. PTCH1 inhibits the Class F G protein-coupled receptor Smoothened (SMO) via a debated mechanism involving modulating accessible cholesterol levels within ciliary membranes. Using extensive molecular dynamics (MD) simulations and free energy calculations to evaluate cholesterol transport through PTCH1, we find an energetic barrier of ~15-20 kJ mol

Identifiers

PMID36824746
PMCPMC9949057
OpenAlexW4320913539

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.