Evidence map›Paper›PMID 36824489›Full record

ArticleBiomaterials and biosystems2022

Transcriptomic Profiling of JEG-3 cells using human leiomyoma derived matrix.

Samineh Barmaki, Ahmed Al-Samadi, Katarzyna Leskinen, Wafa Wahbi, Ville Jokinen, Sanna Vuoristo, Tuula Salo, Juha Kere, Satu Wedenoja, Päivi Saavalainen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials and biosystems, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Samineh BarmakiDepartment of Pharmacology, Faculty of Medicine, University of Helsinki, Helsinki 00290, Finland.
Ahmed Al-SamadiDepartment of Oral and Maxillofacial Disease, University of Helsinki, Helsinki 00290, Finland.
Katarzyna LeskinenTranslational Immunology Research Program, and Department of Clinical and Medical Genetics, University of Helsinki, Helsinki 00290, Finland.
Wafa WahbiDepartment of Pharmacology, Faculty of Medicine, University of Helsinki, Helsinki 00290, Finland.
Ville JokinenDepartment of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo 00076, Finland.
Sanna VuoristoObstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki 00290, Finland.
Tuula SaloDepartment of Oral and Maxillofacial Disease, University of Helsinki, Helsinki 00290, Finland.
Juha KereDepartment of Biosciences and Nutrition, Karolinska Institutet, Huddinge 14183, Sweden.
Satu WedenojaObstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki 00290, Finland.
Päivi SaavalainenTranslational Immunology Research Program, and Department of Clinical and Medical Genetics, University of Helsinki, Helsinki 00290, Finland.
University of Helsinki · FIAalto University · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxygen tension varies during placental and fetal development. Although hypoxia drives early trophoblast invasion, low placental oxygen levels during pregnancy show association with pregnancy complications including fetal growth restriction and preeclampsia. JEG-3 cells are often used as a trophoblast model. We studied transcriptional changes of JEG-3 cells on a uterine leiomyoma derived matrix Myogel. This might be the closest condition to the real uterine environment that we can get for an in vitro model. We observed that culturing JEG-3 cells on the leiomyoma matrix leads to strong stimulation of ribosomal pathways, energy metabolism, and ATP production. Furthermore, Myogel improved JEG-3 cell adherence in comparison to tissue culture treated plastic. We also included PDMS microchip hypoxia creation, and observed changes in oxidative phosphorylation, oxygen related genes and several hypoxia genes. Our study highlights the effects of Myogel matrix on growing JEG-3 cells, especially on mitochondria, energy metabolism, and protein synthesis.

Indexed as

BiomaterialsHypoxiaJEG-3MicroenvironmentMicrofluidicMyogelOxygen scavengerPDMSPlacentaPre-eclampsia

Identifiers

PMID36824489
PMCPMC9934486
OpenAlexW4283072779

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.