Evidence map›Paper›PMID 36824328›Full record

ArticleGigaByte (Hong Kong, China)2021

Improvements in the sequencing and assembly of plant genomes.

Priyanka Sharma, Othman Al-Dossary, Bader Alsubaie, Ibrahim Al-Mssallem, Onkar Nath, Neena Mitter, Gabriel Rodrigues Alves Margarido, Bruce Topp, Valentine Murigneux, Ardashir Kharabian Masouleh and 2 more

Abstract readComment
In one paragraph

Article in GigaByte (Hong Kong, China), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
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  7. Review
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  9. Article
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  11. De novo chromosome level assembly of a plant genome from long read sequence data.The Plant journal : for cell and molecular biology · 2022
    Article
  12. Article
  13. Article
  14. The genome of the endangeredPlant direct · 2021
    Article
  15. Article
  16. The jojoba genome reveals wide divergence of the sex chromosomes in a dioecious plant.The Plant journal : for cell and molecular biology · 2021
    Article
  17. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Priyanka SharmaQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-8000-6415
Othman Al-DossaryQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-1625-3710
Bader AlsubaieQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0003-2693-5480
Ibrahim Al-MssallemCollege of Agriculture and Food Sciences, King Faisal University, Al Hofuf, Saudi Arabia.ORCID https://orcid.org/0000-0002-1489-7547
Onkar NathQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-6348-364X
Neena MitterQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0001-6146-6179
Gabriel Rodrigues Alves MargaridoQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-2327-0201
Bruce ToppQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0001-5647-3737
Valentine MurigneuxGenome Innovation Hub, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-1235-9462
Ardashir Kharabian MasoulehQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-6659-1363
Agnelo FurtadoQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0001-6130-9026
Robert J HenryQueensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane 4072, Australia.ORCID https://orcid.org/0000-0002-4060-0292

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advances in DNA sequencing have made it easier to sequence and assemble plant genomes. Here, we extend an earlier study, and compare recent methods for long read sequencing and assembly. Updated Oxford Nanopore Technology software improved assemblies. Using more accurate sequences produced by repeated sequencing of the same molecule (Pacific Biosciences HiFi) resulted in less fragmented assembly of sequencing reads. Using data for increased genome coverage resulted in longer contigs, but reduced total assembly length and improved genome completeness. The original model species,

Identifiers

PMID36824328
PMCPMC9631998

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.