Evidence map›Paper›PMID 36824271›Full record

ArticleiScience2023

Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics.

Béatrice Corre, Yassine El Janati Elidrissi, Justine Duval, Mailys Quilhot, Gaëtan Lefebvre, Solène Ecomard, Fabrice Lemaître, Zacarias Garcia, Armelle Bohineust, Erica Russo and 1 more

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In one paragraph

Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Béatrice CorreDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Yassine El Janati ElidrissiDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Justine DuvalDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Mailys QuilhotDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Gaëtan LefebvreDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Solène EcomardDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Fabrice LemaîtreDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Zacarias GarciaDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Armelle BohineustDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Erica RussoDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.
Philippe BoussoDynamics of Immune Responses Unit, Institut Pasteur, Université Paris Cité, Inserm U1223, 75015 Paris, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated contacts. To study the integration of calcium signals, we designed a programmable, multiplex illumination strategy for temporally patterned optogenetics (TEMPO). We found that a single round of calcium elevation was insufficient to promote nuclear factor of activated T cells (NFAT) activity and cytokine production in a T cell line. However, robust responses were detected after a second identical stimulation even when signals were separated by several hours. Our results suggest the existence of a biochemical memory of calcium signals in T cells that favors signal integration during temporally separated contacts and promote cytokine production. As illustrated here, TEMPO is a versatile approach for dissecting temporal integration in defined signaling pathways.

Indexed as

BiochemistryBiological sciencesImmunological methodsImmunology

Identifiers

PMID36824271
PMCPMC9942117

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.