Evidence map›Paper›PMID 36823805›Full record

ArticleOptics express2023

Synchronized strobed phase contrast and fluorescence microscopy: the interlaced standard reimagined.

Basarab G Hosu, Winfield Hill, Aravinthan D Samuel, Howard C Berg

Abstract read
In one paragraph

Article in Optics express, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Torque-generating units of the bacterial flagellar motor are rotary motors.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Basarab G Hosu
Winfield Hill
Aravinthan D Samuel
Howard C Berg

Funding

SENSORY TRANSDUCTION IN BACTERIAL CHEMOTAXISR01AI016478 · NIAID · HARVARD UNIVERSITY · PI BERG, HOWARD C · 1985 to 2019
$14.1M
SENSORY TRANSDUCTION IN BACTERIAL CHEMOTAXISR37AI016478 · NIAID · HARVARD UNIVERSITY · PI BERG, HOWARD C · 1990 to 1999
–
NIAID NIH HHS R01 AI016478NIAID NIH HHS R37 AI016478
6 · The paper itself

Abstract

We propose a simple, cost-effective method for synchronized phase contrast and fluorescence video acquisition in live samples. Counter-phased pulses of phase contrast illumination and fluorescence excitation light are synchronized with the exposure of the two fields of an interlaced camera sensor. This results in a video sequence in which each frame contains both exposure modes, each in half of its pixels. The method allows real-time acquisition and display of synchronized and spatially aligned phase contrast and fluorescence image sequences that can be separated by de-interlacing in two independent videos. The method can be implemented on any fluorescence microscope with a camera port without needing to modify the optical path.

Identifiers

PMID36823805
PMCPMC10018787

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.