ArticleBiotechnology for biofuels and bioproducts2023
Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production.
Article in Biotechnology for biofuels and bioproducts, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Valorising Whey: From Environmental Burden to Bio-Based Production of Value-Added Compounds and Food Ingredients.Foods (Basel, Switzerland) · 2025Review
- Adaptive Laboratory Evolution and Carbon/Nitrogen Imbalance Promote High-Yield Ammonia Release inMicroorganisms · 2025Article
- Iron bioleaching and polymers accumulation by an extreme acidophilic bacterium.Archives of microbiology · 2024Article
- Evaluation of Cellular Responses byMicroorganisms · 2024Article
- Antarctic Soil Metabolomics: A Pilot Study.International journal of molecular sciences · 2023Article
- Interference with Bacterial Conjugation and Natural Alternatives to Antibiotics: Bridging a Gap.Antibiotics (Basel, Switzerland) · 2023Article
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Authors and funding
12 authors.
Funding
Abstract
backgroundEscherichia coli cells are the most frequently used hosts in recombinant protein production processes and mainly require molecules such as IPTG or pure lactose as inducers of heterologous expression. A possible way to reduce the production costs is to replace traditional inducers with waste materials such as cheese whey permeate (CWP). CWP is a secondary by-product generated from the production of the valuable whey proteins, which are obtained from ultrafiltration of cheese whey, a main by-product of the dairy industry, which is rich in lactose.
resultsThe effects of CWP collected from an Italian plant were compared with those of traditional inducers on the production of two model proteins (i.e., green fluorescent protein and the toxic Q55 variant of ataxin-3), in E. coli BL21 (DE3) cells. It was found that the high lactose content of CWP (165 g/L) and the antioxidant properties of its micronutrients (vitamins, cofactors and osmolytes) sustain production yields similar to those obtained with traditional inducers, accompanied by the improvement of cell fitness.
conclusionsCWP has proven to be an effective and low-cost alternative inducer to produce recombinant proteins. Its use thus combines the advantage of exploiting a waste product with that of reducing the production costs of recombinant proteins.
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