ArticleNature methods2023
Miniature three-photon microscopy maximized for scattered fluorescence collection.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 75 citations in OpenAlex.
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- Functional Ultrasound Localization Microscopy on Freely Moving Rats.Research square · 2026Article
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- A dual-color miniature endoscope for calcium imaging in behaving mice.bioRxiv : the preprint server for biology · 2026Article
- High-speed neural imaging with multiplexed miniaturized two-photon microscopy.Cell reports methods · 2025Article
- High-throughput two-photon volumetric brain imaging in freely moving mice.Nature communications · 2025Article
- A cortex-wide multimodal microscope for simultaneous CaNature communications · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- A miniature two-photon microscope detects multiple signals deep in the brains of moving mice.Nature methods · 2025Article
- A versatile miniature two-photon microscope enabling multicolor deep-brain imaging.Nature methods · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- 24-hour simultaneous mPFC's miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors.Scientific data · 2025Article
- Dark-based optical sectioning assists background removal in fluorescence microscopy.Nature methods · 2025Article
- Development of a point-of-care-testing platform: localized surface plasmon resonance biosensor for rapid ABO/Rh blood typing.RSC advances · 2025Article
- Integrating Optics and Parametrically-Resonant Micro-Scanner Design for Large Working Distance Implantable Microscopy.IEEE/ASME transactions on mechatronics : a joint publication of the IEEE Industrial Electronics Society and the ASME Dynamic Systems and Control Division · 2025Article
- Wide-field fluorescence navigation system for efficient miniature multiphoton imaging in freely behaving animals.Neurophotonics · 2025Article
- Simultaneous head-mounted imaging of neural and hemodynamic activities at high spatiotemporal resolution in freely behaving mice.Science advances · 2025Article
- Window into the Brain: In Vivo Multiphoton Imaging.ACS photonics · 2025Review
- In vivo three-photon fluorescence imaging of mouse brain vasculature labeled by Evans blue excited at the NIR-III window.Biomedical optics express · 2025Article
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In deep-tissue multiphoton microscopy, diffusion and scattering of fluorescent photons, rather than ballistic emanation from the focal point, have been a confounding factor. Here we report on a 2.17-g miniature three-photon microscope (m3PM) with a configuration that maximizes fluorescence collection when imaging in highly scattering regimes. We demonstrate its capability by imaging calcium activity throughout the entire cortex and dorsal hippocampal CA1, up to 1.2 mm depth, at a safe laser power. It also enables the detection of sensorimotor behavior-correlated activities of layer 6 neurons in the posterior parietal cortex in freely moving mice during single-pellet reaching tasks. Thus, m3PM-empowered imaging allows the study of neural mechanisms in deep cortex and subcortical structures, like the dorsal hippocampus and dorsal striatum, in freely behaving animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.