Evidence map›Paper›PMID 36823281›Full record

ArticleScientific reports2023

Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2.

Hirofumi Ohga, Koki Shibata, Ryo Sakanoue, Takuma Ogawa, Hajime Kitano, Satoshi Kai, Kohei Ohta, Naoki Nagano, Tomoya Nagasako, Seiichi Uchida and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
4.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 13 citations in OpenAlex.

  1. Genome-Edited Fish in the Field.Current issues in molecular biology · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 4 institutions in 1 country.

Hirofumi OhgaAqua-Bioresource Innovation Center (ABRIC) Karatsu Satellite, Faculty of Agriculture, Kyushu University, Saga, 847-0132, Japan.
Koki ShibataLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Ryo SakanoueLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Takuma OgawaLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Hajime KitanoFishery Third Group, Marine Fisheries Research and Development Center, Japan Fisheries Research and Education Agency (FRA), Kanagawa, 221-8529, Japan.
Satoshi KaiLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Kohei OhtaLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.
Naoki NaganoLaboratory of Aquaculture, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Tomoya NagasakoHuman Interface Laboratory, Factory of Information Science and Electrical Engineering, Kyushu University, Fukuoka, 819-0395, Japan.
Seiichi UchidaHuman Interface Laboratory, Factory of Information Science and Electrical Engineering, Kyushu University, Fukuoka, 819-0395, Japan.
Tetsushi SakumaMolecular Genetics Laboratory, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, 739-8526, Japan.
Takashi YamamotoMolecular Genetics Laboratory, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, 739-8526, Japan.
Sangwan KimLaboratory of Molecular Gene Technics, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
Kosuke TashiroLaboratory of Molecular Gene Technics, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
Satoru KuharaLaboratory of Molecular Gene Technics, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
Koichiro GenPlanning and Coordination Department, Fisheries Technology Institute, FRA, Nagasaki, 851-2213, Japan.
Atushi FujiwaraAquatic Breeding Division, Aquaculture Research Department, Fisheries Technology Institute, FRA, Mie, 516-0193, Japan.
Yukinori KazetoFisheries Technology Institute, Minamiizu Field Station, FRA, Shizuoka, 415-0156, Japan.
Takanori KobayashiAquatic Breeding Division, Aquaculture Research Department, Fisheries Technology Institute, FRA, Kanagawa, 236-8648, Japan.
Michiya MatsuyamaABRIC, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan. rinya_m@agr.kyushu-u.ac.jp.
Kyushu University · JPHiroshima University · JPJapan Fisheries Research and Education Agency · JPUniversity of Miyazaki · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genome editing is a technology that can remarkably accelerate crop and animal breeding via artificial induction of desired traits with high accuracy. This study aimed to develop a chub mackerel variety with reduced aggression using an experimental system that enables efficient egg collection and genome editing. Sexual maturation and control of spawning season and time were technologically facilitated by controlling the photoperiod and water temperature of the rearing tank. In addition, appropriate low-temperature treatment conditions for delaying cleavage, shape of the glass capillary, and injection site were examined in detail in order to develop an efficient and robust microinjection system for the study. An arginine vasotocin receptor V1a2 (V1a2) knockout (KO) strain of chub mackerel was developed in order to reduce the frequency of cannibalistic behavior at the fry stage. Video data analysis using bioimage informatics quantified the frequency of aggressive behavior, indicating a significant 46% reduction (P = 0.0229) in the frequency of cannibalistic behavior than in wild type. Furthermore, in the V1a2 KO strain, the frequency of collisions with the wall and oxygen consumption also decreased. Overall, the manageable and calm phenotype reported here can potentially contribute to the development of a stable and sustainable marine product.

Indexed as

CyprinidaePerciformesAggressionAnimalsGene EditingVasotocinVasotocin

Identifiers

PMID36823281
PMCPMC9950132
OpenAlexW4321617816

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.