ArticleGut microbes
Actively replicating gut bacteria identified by 5-ethynyl-2'-deoxyuridine (EdU) click chemistry and cell sorting.
Article in Gut microbes. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Effects of Antibiotic-Induced Gut Microbiota Dysbiosis on Arsenic Bioaccumulation and Transformation in Mice and Its Potential Mechanisms.Environment & health (Washington, D.C.) · 2026Article
- Discovering microbiota functions via chemical probe incorporation for targeted sequencing.Current opinion in chemical biology · 2025Review
- Dextran sodium sulfate-induced colitis alters the proportion and composition of replicating gut bacteria.mSphere · 2025Article
- Design and synthesis of 3,4-Frontiers in chemistry · 2025Article
- Using click chemistry to study microbial ecology and evolution.ISME communications · 2023Review
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The composition of the intestinal bacterial community is well described, but recent research suggests that the metabolism of these bacteria plays a larger role in health than which species are present. One fundamental aspect of gut bacterial metabolism that remains understudied is bacterial replication. Indeed, there exist few techniques which can identify actively replicating gut bacteria. In this study, we aimed to address this gap by adapting 5-ethynyl-2'-deoxyuridine (EdU) click chemistry (EdU-click), a metabolic labeling method, coupled with fluorescence-activated cell sorting and sequencing (FACS-Seq) to characterize replicating gut bacteria. We first used EdU-click with human gut bacterial isolates and show that many of them are amenable to this technique. We then optimized EdU-click and FACS-Seq for murine fecal bacteria and reveal that
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Registered trials
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