Evidence map›Paper›PMID 36817094›Full record

ArticleBiochemistry and biophysics reports2023

A novel methodology for NETs visualization under light microscopy.

Antonio Muñiz-Buenrostro, Alma Y Arce-Mendoza, Edgar I Montes-Zapata, Rubi C Calderón-Meléndez, Hector A Vaquera-Alfaro, Junior A Huerta-Polina, Maricruz J Montelongo-Rodríguez

Open access · goldAbstract read
In one paragraph

Article in Biochemistry and biophysics reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Antonio Muñiz-BuenrostroDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Alma Y Arce-MendozaDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Edgar I Montes-ZapataDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Rubi C Calderón-MeléndezDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Hector A Vaquera-AlfaroDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Junior A Huerta-PolinaDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Maricruz J Montelongo-RodríguezDepartamento de Medicina Familiar, Hospital Universitario "Dr. José E. González", Universidad Autónoma de Nuevo León, Av. Gonzalitos #235 Col. Mitras Centro, C.P. 64460, Monterrey, Nuevo León, México.
Universidad Autónoma de Nuevo León · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neutrophils are the most abundant leukocytes in the bloodstream and are very important for the resolution of infection. One of the strategies used by neutrophils to eliminate a microorganism is the formation of extracellular traps. Different methods for neutrophil extracellular traps (NETs) visualization have been described along the years, usually requiring the use of a fluorescent, confocal or scanning electron microscope. This research aimed to visualize NETs using light microscopy as another way to study NETs prior to using the more expensive techniques, making NETs research more cost effective. We evaluated neutrophil purity, viability and function by analyzing the formation of NETs comparing DAPI with safranin. When evaluating NETs formation, neutrophils that were not stimulated did not form NETs and when neutrophils were exposed to PMA or

Indexed as

NETsNeutrophilSafranin

Identifiers

PMID36817094
PMCPMC9932730
OpenAlexW4319315916

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.