Rapid genotyping in tomato by VPCR using agarose gel-resolvable InDel markers.

Tirthartha Chattopadhyay, Surabhi Sangam, Shirin Akhtar

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Article in 3 Biotech, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

1.6field-weighted citation impact, top 14% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Genetic Analysis and Fingerprint Construction for Thick-Skinned Melon (Plants (Basel, Switzerland) · 2025
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4 · The record

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5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Tirthartha ChattopadhyayDepartment of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.ORCID 0000-0001-5654-0303

Surabhi SangamDepartment of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.ORCID 0000-0001-6132-439X

Shirin AkhtarDepartment of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.ORCID 0000-0002-8137-9467

Bihar Agricultural University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Insertion/deletion (InDel) markers are second most abundant polymerase chain reaction (PCR)-based molecular markers having enormous applications in genotyping and molecular breeding in different crops. Although standard polymerase chain reaction (PCR) for DNA amplification generally takes ~ 1.5 to 2 h, small amplicons can be effectively generated using dynamic heating and cooling through PCR with "V"-shaped thermal profile (VPCR) in ~ 15 to 20 min. Here, we evaluated the applicability of a partly modified VPCR method for amplifying InDels of tomato genome. Out of the 31 InDel markers tested in 15 diverse tomato genotypes, 29 markers resulted in sharp amplicons, where 26 markers were found to be polymorphic. Using this method, the individual DNA amplification reactions could be completed within ~ 30 min. The method was effective for primers varying in melting temperature ( Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03499-x.

Indexed as

High throughput genotypingInsertion/deletion (InDel) markersMolecular breedingRapid DNA amplification

Identifiers

PMID36816752

DOI10.1007/s13205-023-03499-x

PMCPMC9929007

OpenAlexW4320731156

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.