ArticleCell reports methods2023
High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons.
Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- Selective activation of tetrodotoxin-sensitive sodium channels by AaH-II drives arrhythmogenic late Na+ current in the heart.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2026Article
- High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons.Nature protocols · 2026Review
- Nav1.8, an analgesic target for nonpsychotomimetic phytocannabinoids.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Review
- The evolution of patch-clamp electrophysiology: Robotic, multiplex, and dynamic.Molecular pharmacology · 2025Review
- Sodium currents in naïve mouse dorsal root ganglion neurons: No major differences between sexes.Channels (Austin, Tex.) · 2024Article
- Use of FRET-Sensor 'Mermaid' to Detect Subtle Changes in Membrane Potential of Primary Mouse PASMCs.Cells · 2024Article
- Beyond a Transmission Cable-New Technologies to Reveal the Richness in Axonal Electrophysiology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Compartment-specific regulation of NaCell reports · 2024Article
- Functionally-selective inhibition of threshold sodium currents and excitability in dorsal root ganglion neurons by cannabinol.Communications biology · 2024Article
- From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research.Frontiers in pharmacology · 2023Review
- Transcriptomic analysis and high throughput functional characterization of human induced pluripotent stem cell derived sensory neurons.Neurobiology of pain (Cambridge, Mass.)Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The patch-clamp technique is the gold-standard methodology for analysis of excitable cells. However, throughput of manual patch-clamp is slow, and high-throughput robotic patch-clamp, while helpful for applications like drug screening, has been primarily used to study channels and receptors expressed in heterologous systems. We introduce an approach for automated high-throughput patch-clamping that enhances analysis of excitable cells at the channel and cellular levels. This involves dissociating and isolating neurons from intact tissues and patch-clamping using a robotic instrument, followed by using an open-source Python script for analysis and filtration. As a proof of concept, we apply this approach to investigate the biophysical properties of voltage-gated sodium (Nav) channels in dorsal root ganglion (DRG) neurons, which are among the most diverse and complex neuronal cells. Our approach enables voltage- and current-clamp recordings in the same cell, allowing unbiased, fast, simultaneous, and head-to-head electrophysiological recordings from a wide range of freshly isolated neurons without requiring culturing on coverslips.
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Registered trials
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