Evidence map›Paper›PMID 36814374›Full record

ArticleThe Journal of international medical research2023

Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study.

Duminda Subasinghe, Nathan Acott, Pasyodun Koralage Buddhika Mahesh, Sivasuriya Sivaganesh, Sithum Munasinghe, Mariyan Priyanthi Kumarasinghe, Dharmabandu Nandaveva Samarasekera, Menaka Dilani Samarawickrema Lokuhetty

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of international medical research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Duminda SubasingheDepartment of Surgery, Faculty of Medicine, University of Colombo, University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka.ORCID 0000-0003-1805-1589
Nathan AcottPathWest Laboratory Medicine, Perth, & School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia.
Pasyodun Koralage Buddhika MaheshPostgraduate Institute of Medicine, University of Colombo, Colombo, Sri Lanka.
Sivasuriya SivaganeshDepartment of Surgery, Faculty of Medicine, University of Colombo, University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka.
Sithum MunasingheTranslational Health Research Institute, Western Sydney University, Campbelltown Campus, Campbelltown, New South Wales, Australia.
Mariyan Priyanthi KumarasinghePathWest Laboratory Medicine, Perth, & School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia.
Dharmabandu Nandaveva SamarasekeraDepartment of Surgery, Faculty of Medicine, University of Colombo, University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka.
Menaka Dilani Samarawickrema LokuhettyDepartment of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.
The University of Western Australia · AUNational Hospital of Sri Lanka · LKUniversity of Colombo · LKCamden and Campbelltown Hospitals · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivePositive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HER2 expression using immunohistochemistry (IHC) and silver in situ hybridization (SISH).

methodsDuring this 4-year prospective study, clinicopathological data were collected from participants in the National Hospital of Sri Lanka. HER2 IHC and SISH were performed using commercial reagents. Using chi-square tests, associations of HER2-IHC/SISH with clinicopathological features were analyzed.

resultsOverall, 145 GC patients were included, 69 had gastrectomies and 76 had biopsies. Positive HER2 expression by IHC was associated with age <60 years, high T stage (assessed pathologically in resections and radiologically in biopsies), high nuclear grade, tumor necrosis, mitosis >5/high-power field, with additional perineural invasion and lymphovascular invasion in resections. These features, excluding lymphovascular invasion but including male sex, were associated with HER2 expression by SISH.

conclusionsAge <60 years, high nuclear grade, tumor necrosis, and perineural invasion are associated factors of HER2 status. These could be used to triage GC patients for HER2 status testing in limited resource settings where IHC/SISH analysis is costly.

Indexed as

AdenocarcinomaStomach NeoplasmsErb-b2 Receptor Tyrosine KinasesGene ExpressionHumansImmunohistochemistryIn Situ HybridizationMaleMiddle AgedProspective StudiesSilverSri LankaErb-b2 Receptor Tyrosine KinasesSilverclinicopathological factorsdiagnosisgastric carcinomaHuman epidermal growth factor 2immunohistochemistrysilver in situ hybridization

Identifiers

PMID36814374
PMCPMC9950620
OpenAlexW4321602505

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.