ArticleAMB Express2023
Identification and phylogenetic analysis of Enterococcus isolates using MALDI-TOF MS and VITEK 2.
Article in AMB Express, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- Diagnostic advantage of droplet digital PCR over blood culture in sepsis: a retrospective study of 115 patients.European journal of medical research · 2026Article
- Comprehensive identification of contagious, environmental, and emerging microorganisms associated with bovine mastitis in Northern Minas Gerais, Brazil, using MALDI-TOF mass spectrometry.Veterinary world · 2025Article
- A novel thermostable lytic phage vB_EF_Enf3_CCASU-2024-3 against clinical Enterococcus faecium and Enterococcus faecalis.AMB Express · 2025Article
- Isolation and Characterization of Enterocin-ProducingFoods (Basel, Switzerland) · 2025Article
- Microbiological Evaluation of Local and Imported Raw Beef Meat at Retail Sites in Oman with Emphasis on Spoilage and Pathogenic Psychrotrophic Bacteria.Microorganisms · 2024Article
- Immunogenicity of culture filtrated proteins and whole-cell killed formalin ofOpen veterinary journal · 2024Article
- Isolation and Identification of Non-Journal of fungi (Basel, Switzerland) · 2023Article
- The isolation of rumen enterococci strains along with high potential utilizing cyanide.Scientific reports · 2023Article
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The bacterial genus Enterococcus encompasses 38 species. Two of the most common species are E. faecalis and E. faecium. Recently, however, there has been an increase in clinical reports concerning less prevalent Enterococcus species, such as E. durans, E. hirae, and E. gallinarum. Rapid and accurate laboratory methods are needed to facilitate the identification of all these bacterial species. In the present study, we compared the relative accuracy of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS), VITEK 2, and 16S rRNA gene sequencing using 39 enterococci isolates from dairy samples, and compared the resultant phylogenetic trees. We found that MALDI-TOF MS correctly identified all isolates at the species level except for one, whereas the VITEK 2 system, which is an automated identification system using biochemical characteristics of species, misidentified ten isolates. However, phylogenetic trees constructed from both methods showed all isolates in similar positions. Our results clearly showed that MALDI-TOF MS is a reliable and rapid tool for identifying Enterococcus species with greater discriminatory power than the biochemical assay method of VITEK 2.
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