Evidence map›Paper›PMID 36811150›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2023

A Membrane-Associated Light-Harvesting Model is Enabled by Functionalized Assemblies of Gene-Doubled TMV Proteins.

Jing Dai, Kiera B Wilhelm, Amanda J Bischoff, Jose H Pereira, Michel T Dedeo, Derek M García-Almedina, Paul D Adams, Jay T Groves, Matthew B Francis

Open access · bronzeAbstract read
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Virus nanotechnology for intratumoural immunotherapy.Nature reviews bioengineering · 2024
    Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jing DaiDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-9819-0206
Kiera B WilhelmDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-8781-7739
Amanda J BischoffDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0003-0802-275X
Jose H PereiraTechnology Division, Joint BioEnergy Institute, Emeryville, CA, 94720, USA.ORCID 0000-0002-8456-1774
Michel T DedeoDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.
Derek M García-AlmedinaDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-0679-6930
Paul D AdamsTechnology Division, Joint BioEnergy Institute, Emeryville, CA, 94720, USA.ORCID 0000-0001-9333-8219
Jay T GrovesDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-3037-5220
Matthew B FrancisDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0003-2837-2538
University of California, Berkeley · USLawrence Berkeley National Laboratory · US

Funding

User Training and OutreachP30GM124169 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI PAUL David ADAMS, Gregory L Hura · 2017 to 2026
$28.6M
Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Facile Generation of Protein-Protein Conjugates Using Enzymatic Oxidative Coupling ReactionsR01GM138693 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI FRANCIS, MATTHEW B · 2020 to 2025
$1.5M
High Performance Computing Cluster for Public Health Driven Molecular Science - Core FacilityS10OD023532 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI DURKIN, KATHLEEN A · 2017 to 2017
$434k
NIGMS NIH HHS P30 GM124169NIGMS NIH HHS R01 GM138693NIGMS NIH HHS T32 GM066698NIH HHS S10 OD023532
6 · The paper itself

Abstract

Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of light-harvesting complexes embedded within phospholipid membranes. Artificial light-harvesting models are valuable tools for understanding the structural features underpinning energy absorption and transfer within chromophore arrays. Here, a method for attaching a protein-based light-harvesting model to a planar, fluid supported lipid bilayer (SLB) is developed.  The protein model consists of the tobacco mosaic viral capsid proteins that are gene-doubled to create a tandem dimer (dTMV). Assemblies of dTMV break the facial symmetry of the double disk to allow for differentiation between the disk faces. A single reactive lysine residue is incorporated into the dTMV assemblies for the site-selective attachment of chromophores for light absorption. On the opposing dTMV face, a cysteine residue is incorporated for the bioconjugation of a peptide containing a polyhistidine tag for association with SLBs. The dual-modified dTMV complexes show significant association with SLBs and exhibit mobility on the bilayer. The techniques used herein offer a new method for protein-surface attachment and provide a platform for evaluating excited state energy transfer events in a dynamic, fully synthetic artificial light-harvesting system.

Indexed as

PhotosynthesisProteinsEnergy TransferLipid BilayersLipid BilayersProteinsbioconjugationphotosynthesisprotein engineeringsingle molecule imagingsupported lipid bilayersviral capsid proteins

Identifiers

PMID36811150
PMCPMC13553149
OpenAlexW4321498968

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.