Evidence map›Paper›PMID 36810718›Full record

ArticleJournal of the Association for Research in Otolaryngology : JARO2023

Enhanced Expression of Plasminogen Activators and Inhibitor in the Healing of Tympanic Membrane Perforation in Rats.

Maria Makuszewska, Magdalena Cieślińska, Maria M Winnicka, Bożena Skotnicka, Kazimierz Niemczyk, Tomasz Bonda

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the Association for Research in Otolaryngology : JARO, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 0 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Maria MakuszewskaDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Warsaw, Banacha 1a, 02-097, Warsaw, Poland. mmakuszewska@wum.edu.pl.ORCID 0000-0002-1811-6402
Magdalena CieślińskaDepartment of General and Experimental Pathology, Medical University of Białystok, Mickiewicza 2c, 15-222, Białystok, Poland.
Maria M WinnickaDepartment of General and Experimental Pathology, Medical University of Białystok, Mickiewicza 2c, 15-222, Białystok, Poland.
Bożena SkotnickaDepartment of Pediatric Otolaryngology, Medical University of Białystok, Waszyngtona 17, 15-274, Białystok, Poland.
Kazimierz NiemczykDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Warsaw, Banacha 1a, 02-097, Warsaw, Poland.
Tomasz BondaDepartment of General and Experimental Pathology, Medical University of Białystok, Mickiewicza 2c, 15-222, Białystok, Poland.
Medical University of Białystok · PLMedical University of Warsaw · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The significance of plasminogen activation during the tympanic membrane (TM) healing is known mainly from studies performed on knock-out mice. In the previous study, we reported activation of genes coding proteins of plasminogen activation and inhibition system in rat's TM perforation healing. The aim of the present study was the evaluation of protein products expressed by these genes and their tissue distribution using Western blotting and immunofluorescent method, respectively, during 10-day observation period after injury. Otomicroscopical and histological evaluation were employed to assess the healing process. The expression of urokinase plasminogen activator (uPA) and its receptor (uPAR) were significantly upregulated in the proliferation phase, with subsequent gradual attenuation during remodeling phase of healing process, when keratinocyte migration was weakening. The expression of plasminogen activator inhibitor type 1 (PAI-1) also showed the highest levels during the proliferation phase. The increase of tissue plasminogen activator (tPA) expression was observed during the whole observation period, with the highest activity during the remodeling phase. Immunofluorescence of these proteins was present mainly in migrating epithelium. Our study found that plasminogen activation (uPA, uPAR, tPA) and inhibitory (PAI-1) molecules form a well-structured regulatory system of the epithelial migration that is critical to the healing of TM after its perforation.

Indexed as

Tissue Plasminogen ActivatorTympanic Membrane PerforationAnimalsMicePlasminogenPlasminogen Activator Inhibitor 1RatsUrokinase-Type Plasminogen ActivatorPlasminogenPlasminogen Activator Inhibitor 1Tissue Plasminogen ActivatorUrokinase-Type Plasminogen ActivatorPlasminogen activator inhibitor type 1RatsTissue-type plasminogen activatorTympanic membrane perforationUrokinase-type plasminogen activatorUrokinase-type plasminogen activator receptor

Identifiers

PMID36810718
PMCPMC10121974
OpenAlexW4321434896

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.