Evidence map›Paper›PMID 36810248›Full record

ArticleJCI insight2023

PTPN2 regulates bacterial clearance in a mouse model of enteropathogenic and enterohemorrhagic E. coli infection.

Marianne R Spalinger, Vinicius Canale, Anica Becerra, Ali Shawki, Meli'sa Crawford, Alina N Santos, Pritha Chatterjee, Jiang Li, Meera G Nair, Declan F McCole

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Science advances · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 2 countries.

Marianne R SpalingerDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Vinicius CanaleDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Anica BecerraDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Ali ShawkiDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Meli'sa CrawfordDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Alina N SantosDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Pritha ChatterjeeDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Jiang LiDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Meera G NairDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
Declan F McColeDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California, USA.
University of California, Riverside · US

Funding

A NOVEL ROLE FOR PTPN2 IN INTESTINAL EPITHELIAL BARRIER REGULATIONR01DK091281 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Declan McCole · 2012 to 2026
$5.5M
Tyrosine Phosphatase Regulation of Mucosal Macrophage-Epithelial Cell Cross-talkR01AI153314 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI MCCOLE, DECLAN · 2020 to 2024
$2.5M
Inflammatory Bowel Disease Susceptibility Gene Regulation of AnemiaR21AI152017 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI MCCOLE, DECLAN · 2021 to 2022
$428k
NIAID NIH HHS R01 AI153314NIAID NIH HHS R21 AI152017NIDDK NIH HHS R01 DK091281
6 · The paper itself

Abstract

Macrophages intimately interact with intestinal epithelial cells, but the consequences of defective macrophage-epithelial cell interactions for protection against enteric pathogens are poorly understood. Here, we show that in mice with a deletion in protein tyrosine phosphatase nonreceptor type 2 (PTPN2) in macrophages, infection with Citrobacter rodentium, a model of enteropathogenic and enterohemorrhagic E. coli infection in humans, promoted a strong type 1/IL-22-driven immune response, culminating in accelerated disease but also faster clearance of the pathogen. In contrast, deletion of PTPN2 specifically in epithelial cells rendered the epithelium unable to upregulate antimicrobial peptides and consequently resulted in a failure to eliminate the infection. The ability of PTPN2-deficient macrophages to induce faster recovery from C. rodentium was dependent on macrophage-intrinsic IL-22 production, which was highly increased in macrophages deficient in PTPN2. Our findings demonstrate the importance of macrophage-mediated factors, and especially macrophage-derived IL-22, for the induction of protective immune responses in the intestinal epithelium, and show that normal PTPN2 expression in the epithelium is crucial to allow for protection against enterohemorrhagic E. coli and other intestinal pathogens.

Indexed as

Enterobacteriaceae InfectionsEnterohemorrhagic Escherichia coliEscherichia coli InfectionsProtein Tyrosine Phosphatase, Non-Receptor Type 2AnimalsEpithelial CellsHumansMiceProtein Tyrosine Phosphatase, Non-Receptor Type 2PTPN2 protein, humanPtpn2 protein, mouseBacterial infectionsCellular immune responseGastroenterologyInflammationMacrophages

Identifiers

PMID36810248
PMCPMC9977497
OpenAlexW4321436910

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.