Evidence map›Paper›PMID 36809443›Full record

ArticleInvestigational new drugs2023

In vitro effects of gamma-secretase inhibition in HPV-positive and HPV-negative head and neck squamous cell carcinoma.

Sara Varatanovic, Tobias Maier, Sega Al-Gboore, Stefan Stoiber, Sam Augustine Kandathil, Clemens Quint, Charlotte Brennus, Gregor Heiduschka, Lorenz Kadletz-Wanke, Faris F Brkic

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigational new drugs, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Sara VaratanovicDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Tobias MaierDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Sega Al-GbooreDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Stefan StoiberDepartment of Pathology, Medical University of Vienna, Vienna, Austria.
Sam Augustine KandathilDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Clemens QuintDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Charlotte BrennusDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Gregor HeiduschkaDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Lorenz Kadletz-WankeDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Faris F BrkicDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. faris.brkic@meduniwien.ac.at.
Medical University of Vienna · ATChristian Doppler Laboratory for Thermoelectricity · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNew chemotherapy agents are warranted for head and neck squamous cell carcinoma (HNSCC), particularly for incidence-rising HPV-positive tumors. Based on the evidence of Notch pathway involvement in cancer promotion and progression, we aimed to gain insights into the in vitro antineoplastic effects of gamma-secretase inhibition in HPV-positive and -negative HNSCC models.

methodsAll in vitro experiments were conducted in two HPV-negative (Cal27 and FaDu) and one HPV-associated HNSCC cell line (SCC154). The influence of the gamma-secretase inhibitor PF03084014 (PF) on proliferation, migration, colony forming, and apoptosis was assessed.

resultsWe observed significant anti-proliferative, anti-migratory, anti-clonogenic, and pro-apoptotic effects in all three HNSCC cell lines. Furthermore, synergistic effects with concomitant radiation were observable in the proliferation assay. Interestingly, effects were slightly more potent in the HPV-positive cells.

conclusionWe provided novel insights into the potential therapeutic relevance of gamma-secretase inhibition in HNSCC cell lines in vitro. Therefore, PF may become a viable treatment option for patients with HNSCC, particularly for patients with HPV-induced malignancy. Indeed, further in vitro and in vivo experiments should be conducted to validate our results and decipher the mechanism behind the observed anti-neoplastic effects.

Indexed as

Antineoplastic AgentsCarcinoma, Squamous CellHead and Neck NeoplasmsPapillomavirus InfectionsAmyloid Precursor Protein SecretasesCell Line, TumorHumansSquamous Cell Carcinoma of Head and NeckAmyloid Precursor Protein SecretasesAntineoplastic AgentsGamma-secretase inhibitorHead and neck squamous cell carcinomaNotch pathway

Identifiers

PMID36809443
PMCPMC10140088
OpenAlexW4321435076

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.