Evidence map›Paper›PMID 36809389›Full record

ArticleApplied microbiology and biotechnology2023

Development of a novel double-antibody sandwich quantitative ELISA for detecting SADS-CoV infection.

Liyan Cao, Xiangyu Kong, Yu Zhang, Xuepeng Suo, Xiangtong Li, Yueyue Duan, Cong Yuan, Haixue Zheng, Qi Wang

Open access · bronzeAbstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.6field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Liyan CaoInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Xiangyu KongInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Yu ZhangInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Xuepeng SuoInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Xiangtong LiInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Yueyue DuanInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Cong YuanInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Haixue ZhengState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China. haixuezheng@163.com.
Qi WangInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China. qiwang@caas.cn.ORCID http://orcid.org/0000-0001-6942-2410
Lanzhou Veterinary Research Institute · CNNational Institute of Measurement and Testing Technology · CN

Funding

Sichuan Provincial Administration of Traditional Chinese Medicine 2022YFN0008
6 · The paper itself

Abstract

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an emerging swine enteric alphacoronavirus that can cause acute diarrhea, vomiting, dehydration, and death of newborn piglets. In this study, we developed a double-antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-qELISA) for detection of SADS-CoV by using an anti-SADS-CoV N protein rabbit polyclonal antibody (PAb) and a specific monoclonal antibody (MAb) 6E8 against the SADS-CoV N protein. The PAb was used as the capture antibodies and HRP-labeled 6E8 as the detector antibody. The detection limit of the developed DAS-qELISA assay was 1 ng/mL of purified antigen and 10

Indexed as

AlphacoronavirusCoronavirus InfectionsPorcine epidemic diarrhea virusSwine DiseasesAnimalsEnzyme-Linked Immunosorbent AssayRabbitsSwineAntigen detectionDAS-qELISAMonoclonal antibodyNucleocapsid proteinSwine acute diarrhea syndrome coronavirus (SADS-CoV)

Identifiers

PMID36809389
PMCPMC9942060
OpenAlexW4321437291

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.