ArticleApplied microbiology and biotechnology2023
Development of an efficient ClosTron system for gene disruption in Ruminiclostridium papyrosolvens.
Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Strategies for engineering domesticated and undomesticated human microbes.Current opinion in biotechnology · 2025Review
- Expression of xylanase XynB is synergistically controlled by two two-component systems inApplied and environmental microbiology · 2025Article
- Combining transcriptomic and metabolomic insights into carbohydrate utilization by Ruminiclostridium papyrosolvens DSM2782.Biotechnology for biofuels and bioproducts · 2025Article
- Gallic acid as biofilm inhibitor can improve transformation efficiency of Ruminiclostridium papyrosolvens.Biotechnology letters · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- Insights into lignocellulose degradation: comparative genomics of anaerobic and cellulolyticFrontiers in microbiology · 2023Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Ruminiclostridium papyrosolvens is an anaerobic, mesophilic, and cellulolytic clostridia, promising consolidated bioprocessing (CBP) candidate for producing renewable green chemicals from cellulose, but its metabolic engineering is limited by lack of genetic tools. Here, we firstly employed the endogenous xylan-inducible promoter to control ClosTron system for gene disruption of R. papyrosolvens. The modified ClosTron can be easily transformed into R. papyrosolvens and specifically disrupt targeting genes. Furthermore, a counter selectable system based on uracil phosphoribosyl-transferase (Upp) was successfully established and introduced into the ClosTron system, which resulted in plasmid curing rapidly. Thus, the combination of xylan-inducible ClosTron and upp-based counter selectable system makes the gene disruption more efficient and convenient for successive gene disruption in R. papyrosolvens. KEY POINTS: • Limiting expression of LtrA enhanced the transformation of ClosTron plasmids in R. papyrosolvens. • DNA targeting specificity can be improved by precise management of the expression of LtrA. • Curing of ClosTron plasmids was achieved by introducing the upp-based counter selectable system.
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