Evidence map›Paper›PMID 36807640›Full record

ArticleNature immunology2023

Temporal single-cell atlas of non-neuronal retinal cells reveals dynamic, coordinated multicellular responses to central nervous system injury.

Inbal Benhar, Jiarui Ding, Wenjun Yan, Irene E Whitney, Anne Jacobi, Malika Sud, Grace Burgin, Karthik Shekhar, Nicholas M Tran, Chen Wang and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed.

  1. AAV-NRF2 protects retinal and choroidal vasculature in a GDF15-dependent manner in an oxidative damage model of AMD.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Engulfment by brain macrophages in a short-lived vertebrate.bioRxiv : the preprint server for biology · 2026
    Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. The neuroimmune interface in retinal regeneration.Progress in retinal and eye research · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Inbal Benhar *Broad Institute of MIT and Harvard, Cambridge, MA, USA. inbal.benhar@gmail.com.ORCID 0000-0002-0239-2162
Jiarui Ding *Broad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0000-0003-3670-7764
Wenjun YanDepartment of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA, USA.ORCID 0000-0003-3568-4265
Irene E WhitneyDepartment of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA, USA.
Anne JacobiDepartment of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA, USA.
Malika SudBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0000-0001-9258-7029
Grace BurginBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Karthik ShekharBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Nicholas M TranDepartment of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA, USA.
Chen WangF.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
Zhigang HeF.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-6080-6880
Joshua R SanesDepartment of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, MA, USA.
Aviv RegevBroad Institute of MIT and Harvard, Cambridge, MA, USA. aviv.regev.sc@gmail.com.ORCID 0000-0003-3293-3158

Funding

SOCS3 and optic nerve regenerationR01EY021526 · NEI · BOSTON CHILDREN'S HOSPITAL · PI HE, ZHIGANG · 2011 to 2020
$4.1M
Uncovering the molecular identity of retinal cell types, and their responses to nerve injury using single-cell transcriptomicsR00EY028625 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI SHEKHAR, KARTHIK · 2020 to 2022
$740k
NEI NIH HHS R01 EY021526
6 · The paper itself

Abstract

Non-neuronal cells are key to the complex cellular interplay that follows central nervous system insult. To understand this interplay, we generated a single-cell atlas of immune, glial and retinal pigment epithelial cells from adult mouse retina before and at multiple time points after axonal transection. We identified rare subsets in naive retina, including interferon (IFN)-response glia and border-associated macrophages, and delineated injury-induced changes in cell composition, expression programs and interactions. Computational analysis charted a three-phase multicellular inflammatory cascade after injury. In the early phase, retinal macroglia and microglia were reactivated, providing chemotactic signals concurrent with infiltration of CCR2

Indexed as

MacrophagesRetinaAnimalsCentral Nervous SystemMiceMicrogliaMonocytes

Identifiers

PMID36807640

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.