ArticleNature communications2023
The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- A bacteriophage split DNA polymerase incorporates a sliding clamp-derived processivity subunit.bioRxiv : the preprint server for biology · 2026Article
- Article
- The biology of jumbo phages.Nature communications · 2026Review
- Molecular basis for anti-jumbo phage immunity by AVAST type 5.Molecular cell · 2026Article
- Bacteriophage genome-wide transposon mutagenesis.bioRxiv : the preprint server for biology · 2025Article
- The complex developmental mechanisms of nucleus-forming jumbo phages.Current opinion in microbiology · 2025Review
- The Biology of Nucleus-Forming Jumbo Phages.Annual review of genetics · 2025Review
- Article
- Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.Cell host & microbe · 2025Article
- Deconvolution to restore cryo-EM maps with anisotropic resolution.Research square · 2025Article
- Deconvolution to restore cryo-EM maps with anisotropic resolution.bioRxiv : the preprint server for biology · 2025Article
- Article
- Erwinia phage Asesino is a nucleus-forming phage that lacks PhuZ.Scientific reports · 2025Article
- Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024Review
- Genomic transfer via membrane vesicle: a strategy of giant phage phiKZ for early infection.Journal of virology · 2024Article
- A transcriptionally active lipid vesicle encloses the injectedbioRxiv : the preprint server for biology · 2024Article
- Exploring pangenomic diversity and CRISPR-Cas evasion potential in jumbo phages: a comparative genomics study.Microbiology spectrum · 2024Article
- Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection.Cell host & microbe · 2024Article
- Review
- The intricate organizational strategy of nucleus-forming phages.Current opinion in microbiology · 2024Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
To protect themselves from host attack, numerous jumbo bacteriophages establish a phage nucleus-a micron-scale, proteinaceous structure encompassing the replicating phage DNA. Bacteriophage and host proteins associated with replication and transcription are concentrated inside the phage nucleus while other phage and host proteins are excluded, including CRISPR-Cas and restriction endonuclease host defense systems. Here, we show that nucleus fragments isolated from ϕPA3 infected Pseudomonas aeruginosa form a 2-dimensional lattice, having p2 or p4 symmetry. We further demonstrate that recombinantly purified primary Phage Nuclear Enclosure (PhuN) protein spontaneously assembles into similar 2D sheets with p2 and p4 symmetry. We resolve the dominant p2 symmetric state to 3.9 Å by cryo-EM. Our structure reveals a two-domain core, organized into quasi-symmetric tetramers. Flexible loops and termini mediate adaptable inter-tetramer contacts that drive subunit assembly into a lattice and enable the adoption of different symmetric states. While the interfaces between subunits are mostly well packed, two are open, forming channels that likely have functional implications for the transport of proteins, mRNA, and small molecules.
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