Evidence map›Paper›PMID 36807063›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Measuring Inaccessible Chromatin Genome-Wide Using Protect-seq.

George Spracklin, Liyan Yang, Sriharsa Pradhan, Job Dekker

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

George SpracklinProgram in Systems Biology, University of Massachusetts Medical School, Worcester, MA, USA. george.spracklin@umassmed.edu.
Liyan YangProgram in Systems Biology, University of Massachusetts Medical School, Worcester, MA, USA.
Sriharsa PradhanNew England Biolabs, Ipswich, MA, USA.
Job DekkerProgram in Systems Biology, University of Massachusetts Medical School, Worcester, MA, USA.

Funding

Center for 3D Structure and Physics of the GenomeU54DK107980 · NIDDK · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI DEKKER, JOB · 2015 to 2019
$14.9M
Center for 3D Structure and Physics of the GenomeUM1HG011536 · NHGRI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI DEKKER, JOB, MIRNY, LEONID A · 2020 to 2024
$11.8M
NHGRI NIH HHS UM1 HG011536NIDDK NIH HHS U54 DK107980
6 · The paper itself

Abstract

Chromatin accessibility has been an immensely powerful metric for identifying and understanding regulatory elements in the genome. Many important regulatory elements, such as enhancers and transcriptional start sites, are characterized by "open" or nucleosome-free regions. Understanding the areas of the genome that are not considered open chromatin has been more difficult. Protect-seq is a genomics technique that aims to identify inaccessible chromatin associated with the nuclear periphery. These regions are enriched for histone modifications associated with transcriptional repression and correlate with loci identified by other techniques measuring heterochromatin and peripheral localization. Here, we discuss the protocol and best practices to perform Protect-seq.

Indexed as

ChromatinNucleosomesGenomeHeterochromatinRegulatory Sequences, Nucleic AcidChromatinHeterochromatinNucleosomesChromatinGenomicsHeterochromatinNuclear peripheryProtect-seq

Identifiers

PMID36807063

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.