ArticleClinical oral investigations2023
Differential analysis of culturable and unculturable subgingival target microorganisms according to the stages of periodontitis.
Article in Clinical oral investigations, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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15 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Pooled it
- Culturable and unculturable periodontal microorganisms in non-small cell lung cancer: an exploratory analysis.Scientific reports · 2026Article
- Salivary microbiota signatures of periodontitis are associated with CGM-derived short-term glycaemic control in adults with type 1 diabetes: a pilot study.Frontiers in endocrinology · 2026Article
- Unveiling potential driver taxa in subgingival plaque and their roles in mediating periodontitis progression.Journal of oral microbiology · 2026Article
- Mechanisms underlying the role of the oral-gut-liver axis in the promotion of liver fibrosis by periodontal disease: an interacting network of inflammation, microbiota, and barrier function.Frontiers in cellular and infection microbiology · 2026Review
- Peri-implantitis biofilm from explanted implants in Korean patients: microbial and functional profiling.Frontiers in cellular and infection microbiology · 2026Article
- Oral microbiome dynamics across periodontitis severity stratified by type 2 diabetes status.Journal of oral microbiology · 2026Article
- Peridontopathogenic key species in correlation to the current classification system.Clinical oral investigations · 2025Article
- Emerging oralFrontiers in oral health · 2025Article
- Tobacco-enhanced biofilm formation by Porphyromonas gingivalis and other oral microbes.Molecular oral microbiology · 2024Review
- Exploring the Efficacy of Novel Therapeutic Strategies for Periodontitis: A Literature Review.Life (Basel, Switzerland) · 2024Review
- Oral microbiome associated with differential ratios ofMicrobiology spectrum · 2024Article
- Filifactor alocis enhances survival of Porphyromonas gingivalis W83 in response to HMolecular oral microbiology · 2024Article
- Factors Associated with the Extent of Clinical Attachment Loss in Periodontitis: A Multicenter Cross-Sectional Study.International journal of environmental research and public health · 2023Article
- Hypochlorous Acid as a Potential Postsurgical Antimicrobial Agent in Periodontitis: A Randomized, Controlled, Non-Inferiority Trial.Antibiotics (Basel, Switzerland) · 2023Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesCulturable and unculturable microorganisms have been associated with periodontitis. Their differential proportions and composition have not been evaluated by their severity and complexity defined by stages in the 2018 AAP-EEP classification.
methodsOne hundred eighty subgingival biofilm samples were collected in Spain and Colombia from subjects categorized as health/gingivitis: periodontitis stages I/II periodontitis stages III/IV. Target culturable microorganisms (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Treponema denticola, and Eubacterium nodatum) and target unculturable microorganisms (Filifactor alocis, Eubacterium saphenum, Eubacterium brachy, Desulfobulbus oralis) were evaluated by quantitative PCR analysis. In addition, their differences and association with periodontal status were analyzed by ANCOVA and logistic regression models once adjusted to age, current smoking, and country.
resultsP. gingivalis was significantly associated with periodontitis stages I/II, OR 2.44 (CI 95% 1.08-5.47) and stages III/V, OR 6.43 (CI 95% 2.43-16.9). T forsythia, OR 7.53 (CI 95% 2.07-27.4); D. oralis, OR 5.99 (CI 95% 2.71-13.23); F. alocis, OR 10.9 (CI 95% 4.56-23.2); E. brachy, 3.57 (CI 95% 1.40-9.11); and E. saphenum, 4.85 (CI 95% 1.99-11.7) were significantly associated only with stages III/IV periodontitis. P. gingivalis evidenced significant differences with the increase in the severity of the periodontal lesion: 2.97 colony forming unit (CFU)/μL (CI 95% 2.32-3.54) health/gingivitis, and 4.66 CFU/μL (CI 95% 4.03-5.30) and 5.90 CFU/μL (CI 95% 5.20-6.48) in stages I/II and III/IV respectively (p < 0.0001). Unculturable microorganisms only evidenced differences in concentration in stages III/IV compared with health-gingivitis (p ≤ 0.001).
conclusionCulturable and unculturable are strongly associated with stages III/IV periodontitis. Classic culturable microorganisms are more sensitive to differentiate between stages of periodontitis in the quantitative analysis. CLINICAL RELEVANCE: Future interventional studies of periodontal disease should include Filifactor alocis, Eubacterium saphenum, Eubacterium brachy, and Desulfobulbus oralis as possible markers of therapy response and as indicators of progressive disease.
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