Evidence map›Paper›PMID 36806671›Full record

ArticleExperimental eye research2023

Effect of graphene-based nanomaterials on corneal wound healing in vitro.

Atsuhiko Fukuto, Jennifer Kang, Brooke L Gates, Kimberley Sannajust, Kent E Pinkerton, Laura S Van Winkle, Yoshiaki Kiuchi, Brian C Leonard, Sara M Thomasy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Atsuhiko FukutoDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA; Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Minami-ku, Kasumi 1-2-3, Hiroshima, 734-8551, Japan.
Jennifer KangDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.
Brooke L GatesDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.
Kimberley SannajustDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.
Kent E PinkertonCenter for Health and the Environment, University of California-Davis, Davis, CA, 95616, USA; Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.
Laura S Van WinkleCenter for Health and the Environment, University of California-Davis, Davis, CA, 95616, USA; Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.
Yoshiaki KiuchiDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Minami-ku, Kasumi 1-2-3, Hiroshima, 734-8551, Japan.
Brian C LeonardDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.
Sara M ThomasyDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA; Department of Ophthalmology & Vision Science, School of Medicine, University of California-Davis, Davis, CA, 95616, USA. Electronic address: smthomasy@ucdavis.edu.
University of California, Davis · USHiroshima University · JP

Funding

VISION RESEARCH CORE GRANTP30EY012576 · NEI · UNIVERSITY OF CALIFORNIA DAVIS · PI LISA M OAKES · 1999 to 2026
$18.9M
Engineered Nanomaterial Synthesis, Characterization and Method Development Center for Nano-safety ResearchU24ES026946 · NIEHS · HARVARD SCHOOL OF PUBLIC HEALTH · PI DEMOKRITOU, PHILIP · 2016 to 2020
$3.8M
Biophysical Cues and Corneal Wound HealingR01EY019970 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MURPHY, CHRISTOPHER JOHN · 2011 to 2020
$3.5M
Respiratory and Ocular Toxicity of Inhaled NanomaterialsU01ES027288 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PINKERTON, KENT ED, THOMASY, SARA MICHELLE · 2016 to 2020
$2.2M
Modulation of Hsp90 signaling to limit corneal fibrosis and improve ocular drug penetrationK08EY028199 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEONARD, BRIAN C. · 2017 to 2021
$1.1M
NEI NIH HHS K08 EY028199NEI NIH HHS P30 EY012576NEI NIH HHS R01 EY019970NIEHS NIH HHS U01 ES027288NIEHS NIH HHS U24 ES026946
6 · The paper itself

Abstract

Graphene-based nanomaterials (GBNs) are widely used due to their chemical and physical properties for multiple commercial and environmental applications. From an occupational health perspective, there is concern regarding the effects of inhalation on the respiratory system, and many studies have been conducted to study inhalation impacts on lung. Similar to the respiratory system, the eyes may also be exposed to GBNs and thus impacted. In this study, immortalized human corneal epithelial (hTCEpi) cells and rabbit corneal fibroblasts (RCFs) were used to investigate the toxicity of eight types of GBN: graphene oxide (GO; 400 nm), GO (1 μm), partially reduced graphene oxide (PRGO; 400 nm), reduced graphene oxide (RGO; 400 nm), RGO (2 μm), graphene (110 nm), graphene (140 nm), and graphene (1 μm). We next examined the effects of these GBNs on hTCEpi cell migration. We also determined whether the expression of α-smooth muscle actin (αSMA), a myofibroblast marker, is altered by the GBNs using RCFs. We found that RGO (400 nm) and RGO (2 μm) were highly toxic to hTCEPi cells and RCFs meanwhile, PRGO (400 nm) was toxic only to hTCEpi cells. In addition, PRGO (400 nm), RGO (400 nm), and RGO (2 μm) inhibited hTCEpi cell migration and significantly increased αSMA mRNA expression. Further study in vivo is required to determine if RGO nanomaterials delay corneal epithelial healing and induce scar formation.

Indexed as

GraphiteNanostructuresAnimalsCorneaHumansRabbitsWound Healinggraphene oxideGraphiteCorneal wound healingGraphene-based nanomaterialsKeratocyte-fibroblast-myofibroblast transformation

Identifiers

PMID36806671
PMCPMC10131158
OpenAlexW4321113866

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.