ArticleScientific reports2023
Molecular mechanism of valine and its metabolite in improving triglyceride synthesis of porcine intestinal epithelial cells.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.Molecular nutrition & food research · 2026Article
- Increased maternal valine intake in low-protein diets accelerates growth performance of suckling piglets by improving mammary gland-fetal intestinal metabolism.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Beyond Protein Building Blocks: A Review of Biological Roles and Therapeutic Potential of Free Amino Acids.International journal of molecular sciences · 2025Review
- Integrating omics and functional data via representation learning to prioritize candidate genes for pleiotropic effect in dairy sheep.PNAS nexus · 2025Article
- Reduction in within-litter variation of piglet birth weight through dietary supplementation of 3-hydroxyisobutyric acid in sows.Frontiers in veterinary science · 2025Article
- Maternal gut Bifidobacterium breve modifies fetal brain metabolism in germ-free mice.Molecular metabolism · 2024Article
- Valine metabolite, 3-hydroxyisobutyrate, promotes lipid metabolism and cell proliferation in porcine mammary gland epithelial cells.Frontiers in nutrition · 2024Article
- Valine induces inflammation and enhanced adipogenesis in lean mice by multi-omics analysis.Frontiers in nutrition · 2024Article
- The biological functions and metabolic pathways of valine in swine.Journal of animal science and biotechnology · 2023Review
- Impaired Adipocyte SLC7A10 Promotes Lipid Storage in Association With Insulin Resistance and Altered BCAA Metabolism.The Journal of clinical endocrinology and metabolism · 2023Article
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Authors and funding
9 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
An insufficient energy supply to intestinal epithelial cells decreases production performance in weaned piglets. Triglycerides are the main energy source for intestinal epithelial cells in piglets. The present study aimed to investigate the effects and mechanisms of valine supplementation on triglyceride synthesis in porcine intestinal epithelial (IPEC-J2) cells. Valine supplementation in the medium significantly increased the content of triglycerides, fat droplets, and long-chain fatty acids (C17:0, C18:0, C20:0, C18:1, C20:1, and C22:1) (P < 0.05). Valine metabolite (3-hydroxyisobutyrate [3-HIB]) concentration increased significantly in the valine-supplemented group (P < 0.05). Silencing of the 3-HIB synthase enzyme 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) in IPEC-J2 cells significantly reduced the triglyceride concentration and lipid droplet synthesis. Further studies found that 3-HIB supplementation in the medium significantly increased the concentration of triglycerides, lipid droplets, and unsaturated fatty acids (C16:1, C18:1, C18:2, C18:3, C20:3, C20:4, and C20:5) (P < 0.05) by upregulating the expression of proteins involved in fatty acid transport (CD36) and fatty acid binding protein 3 (FABP3) or triglyceride synthesis (DGAT1) (P < 0.05), indicating that 3-HIB mediates valine-enhanced triglyceride synthesis in IPEC-J2 cells. In conclusion, our results demonstrated that valine enhanced triglyceride synthesis in IPEC-J2 cells via increasing the 3-HIB concentration, which may promote fatty acid transport via upregulation of proteins related to fatty acid transporter. These findings provide new insights into the mechanisms through which valine participates in lipid metabolism.
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