ArticleeLife2023
Cryo-plasma FIB/SEM volume imaging of biological specimens.
Article in eLife, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Integrating in situ single-particle cryo-electron microscopy with cryo-electron tomography for high-resolution structural biology in native cellular contexts.Current opinion in structural biology · 2026Review
- Beyond structure: spectroscopic imaging in cryogenic electron microscopy.Current opinion in structural biology · 2026Review
- Adding chemical identity to cryo-electron microscopy.Nature methods · 2026Article
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- To Dye or Not to Dye: Unraveling the Impact of Surface Chemistry on Cerium Oxide Nanoparticles-Cell Interactions.Small science · 2026Article
- Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway.Nature reviews. Molecular cell biology · 2025Review
- A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue.Cell reports methods · 2025Article
- Structural glycobiology - from enzymes to organelles.Biochemical Society transactions · 2025Review
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Methods to Study Poxvirus Structures by Cryo-EM Imaging Modalities.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cryo-electron tomography: en route to the molecular anatomy of organisms and tissues.Biochemical Society transactions · 2024Review
- Bridging structural biology and clinical research through in-tissue cryo-electron tomography.The EMBO journal · 2024Article
- Zooming in and out: Exploring RNA Viral Infections with Multiscale Microscopic Methods.Viruses · 2024Review
- Serial Lift-Out: sampling the molecular anatomy of whole organisms.Nature methods · 2024Article
- Cryopreservation of brain cell structure: a review.Free neuropathology · 2024Article
- Structural brain preservation: a potential bridge to future medical technologies.Frontiers in medical technology · 2024Article
- Integrative Approaches to Study Virus Structures.Sub-cellular biochemistry · 2024Review
- Cryo-electron tomography to study viral infection.Biochemical Society transactions · 2023Review
- The 3D Controllable Fabrication of Nanomaterials with FIB-SEM Synchronization Technology.Nanomaterials (Basel, Switzerland) · 2023Review
- Plasma FIB milling for the determination of structures in situ.Nature communications · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors at 5 institutions in 3 countries.
Funding
Abstract
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures in cells and tissues while maintaining constituents' hydration shells for in situ structural biology downstream. However, the application of serial FIB/SEM imaging of non-stained cryogenic biological samples is limited due to low contrast, curtaining, and charging artefacts. We address these challenges using a cryogenic plasma FIB/SEM. We evaluated the choice of plasma ion source and imaging regimes to produce high-quality SEM images of a range of different biological samples. Using an automated workflow we produced three-dimensional volumes of bacteria, human cells, and tissue, and calculated estimates for their resolution, typically achieving 20-50 nm. Additionally, a tag-free localisation tool for regions of interest is needed to drive the application of in situ structural biology towards tissue. The combination of serial FIB/SEM with plasma-based ion sources promises a framework for targeting specific features in bulk-frozen samples (>100 µm) to produce lamellae for cryogenic electron tomography.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.