ArticleThe Journal of cell biology1987
Structure and assembly of desmosome junctions: biosynthesis and turnover of the major desmosome components of Madin-Darby canine kidney cells in low calcium medium.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 78 citations in OpenAlex.
- Pitfalls in the Application of Dispase-Based Keratinocyte Dissociation Assay for In Vitro Analysis of Pemphigus Vulgaris.Vaccines · 2022Article
- Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization Microscopy.Biophysical journal · 2017Article
- ER-to-Golgi blockade of nascent desmosomal cadherins in SERCA2-inhibited keratinocytes: Implications for Darier's disease.Traffic (Copenhagen, Denmark) · 2017Article
- Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation.Molecular biology of the cell · 2014Article
- Chemical genetic screen reveals a role for desmosomal adhesion in mammary branching morphogenesis.The Journal of biological chemistry · 2013Article
- Article
- Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes.The Journal of cell biology · 2011Article
- Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton.Experimental cell research · 2011Article
- Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.The Journal of cell biology · 2011Article
- Intercellular junction assembly, dynamics, and homeostasis.Cold Spring Harbor perspectives in biology · 2010Review
- Desmosomes in vivo.Dermatology research and practice · 2010Article
- Desmocollin switching in colorectal cancer.British journal of cancer · 2006Article
- Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.The Journal of cell biology · 2005Article
- Assessment of splice variant-specific functions of desmocollin 1 in the skin.Molecular and cellular biology · 2004Article
- Continual assembly of half-desmosomal structures in the absence of cell contacts and their frustrated endocytosis: a coordinated Sisyphus cycle.The Journal of cell biology · 1995Article
- Remodeling the cell surface distribution of membrane proteins during the development of epithelial cell polarity.The Journal of cell biology · 1992Article
- Epithelial cell adhesion mechanisms.The Journal of membrane biology · 1989Review
- Rearrangements of desmosomal and cytoskeletal proteins during the transition from epithelial to fibroblastoid organization in cultured rat bladder carcinoma cells.The Journal of cell biology · 1989Article
- Regulation of desmosome assembly in epithelial cells: kinetics of synthesis, transport, and stabilization of desmoglein I, a major protein of the membrane core domain.The Journal of cell biology · 1989Article
- Organization of desmosomal plaque proteins in cells growing at low calcium concentrations.The Journal of cell biology · 1988Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neither stratifying (primary keratinocytes) nor simple (Madin-Darby canine kidney [MDCK] and Madin-Darby bovine kidney [MDBK]) epithelial cell types from desmosomes in low calcium medium (LCM; less than 0.1 mM), but they can be induced to do so by raising the calcium level to physiological concentrations (standard calcium medium [SCM], 2 mM). We have used polyclonal antisera to the major bovine epidermal desmosome components (greater than 100 kD) in a sensitive assay involving immunoprecipitation of the components from metabolically labeled MDCK cell monolayers to investigate the mechanism of calcium-induced desmosome formation. MDCK cells, whether cultured in LCM or SCM, were found to synthesize the desmosome protein, DPI and desmosome glycoproteins DGI and DGII/III with identical electrophoretic mobility, and also, where relevant, with similar carbohydrate addition/processing and proteolytic processing. The timings of these events and of transport of DGI to the cell surface were similar in low and high calcium. Although the rates of synthesis of the various desmosome components were also similar under both conditions, the glycoprotein turnover rates increased dramatically in cells cultured in LCM. The half-lives decreased by a factor of about 7 for DGI and 12 for DGII/III and, consistent with this, MDCK cells labeled for 48 h in SCM had three and six times the amount of DGI and DGII/III, respectively, as cells labeled for 48 h in LCM. The rate of turnover and the levels of DPI were changed in the same direction, but to much lesser extents. Possible mechanisms for the Ca2+-dependent control of desmosome formation are discussed in the light of this new evidence.
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