ArticleBiochemistry2023
How Sugars Protect Dry Protein Structure.
Article in Biochemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
- Polypeptide Sequence Effects on Virus Thermostability in Complex Coacervate Formulations.Biomacromolecules · 2026Article
- Mechanochemical Production of Oligosaccharide Alcohols via Borohydride-Mediated Activation and Reductive Stabilization.ChemSusChem · 2026Article
- Monomeric Insulin Analog and Stabilizing Excipient Enabled Ultrafast Insulin Formulation.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Drying parameters and aging modulate protective properties of vitrified trehalose.bioRxiv : the preprint server for biology · 2026Article
- Drying parameters and aging modulate protective properties of vitrified trehalose.BBA advances · 2026Article
- Characterization and storage stability of microencapsulatedFood chemistry: X · 2025Article
- A Versatile Enzymatic Pathway for Modification of Peptide C‑Termini.ACS central science · 2025Article
- Anti-Pseudomonas Aeruginosa Bacteriophage Loaded Electrospun Fibers for Antibacterial Wound Dressings.Macromolecular rapid communications · 2025Article
- Biomolecular condensates-Prerequisites for anhydrobiosis?Protein science : a publication of the Protein Society · 2025Review
- Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches.The Journal of clinical investigation · 2025Review
- Understanding dry proteins and their protection with solid-state hydrogen-deuterium exchange.Protein science : a publication of the Protein Society · 2025Review
- A Design of Experiments Approach for Enhancing Room Temperature Stability of a Lyophilised and Paper-Based Bacterial Cell-Free System.Bioengineering (Basel, Switzerland) · 2025Article
- Sustained Delivery of Chondrogenic Molecules Using Sugar Glass Nanoparticle-Integrated Fibrous Scaffolds for Cartilage Tissue Engineering.Regenerative engineering and translational medicine · 2025Article
- Crowding in Anhydrobiosis.Sub-cellular biochemistry · 2025Review
- Trehalose Interferes with the Photosynthetic Electron Transfer Chain ofInternational journal of molecular sciences · 2024Article
- Effectiveness of Lyoprotectants in Protein Stabilization During Lyophilization.Pharmaceutics · 2024Review
- Trimethylamine N-oxide (TMAO) doubly locks the hydrophobic core and surfaces of protein against desiccation stress.Protein science : a publication of the Protein Society · 2024Article
- A Structural Study on Absorption of Lysozyme in Amorphous Starch Microspheres.Molecular pharmaceutics · 2024Article
- Colyophilized Sugar-Polymer Dispersions for Enhanced Processing and Storage Stability.Molecular pharmaceutics · 2024Article
- Protecting Proteins from Desiccation Stress Using Molecular Glasses and Gels.Chemical reviews · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Extremotolerant organisms and industry exploit sugars as desiccation protectants, with trehalose being widely used by both. How sugars, in general, and the hydrolytically stable sugar trehalose, in particular, protect proteins is poorly understood, which hinders the rational design of new excipients and implementation of novel formulations for preserving lifesaving protein drugs and industrial enzymes. We employed liquid-observed vapor exchange nuclear magnetic resonance (LOVE NMR), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA) to show how trehalose and other sugars protect two model proteins: the B1 domain of streptococcal protein G (GB1) and truncated barley chymotrypsin inhibitor 2 (CI2). Residues with intramolecular H-bonds are most protected. The LOVE NMR and DSC data indicate that vitrification may be protective. Combining LOVE NMR and TGA data shows that water retention is not important. Our data suggest that sugars protect protein structure as they dry by strengthening intraprotein H-bonds and water replacement and that trehalose is the stress-tolerance sugar of choice because of its covalent stability.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.