ArticlePLoS neglected tropical diseases2023
Evaluating the efficacy of serological testing of clinical specimens collected from patients with suspected brucellosis.
Article in PLoS neglected tropical diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 24 citations in OpenAlex.
- Brucellosis Seroprevalence, Analysis of Risk Factors, and Comparison of Test Methods Used in Diagnosis in a Tertiary Hospital in Kahramanmaraş.Tropical medicine and infectious disease · 2026Article
- Prevalence and detection ofFrontiers in cellular and infection microbiology · 2026Article
- Evaluation of Brucella IgM and IgG ELISA Responses in Relation to Blood Culture Positivity in Pediatric Brucellosis.Infection and drug resistance · 2026Article
- Two Years, Many Clues: Diagnostic Journey and Treatment of Chronic Neurobrucellosis in an Elderly Woman.Cureus · 2025Article
- Bioinformatic analysis of brucellosis and construction of a diagnostic model based on key genes.Scientific reports · 2025Article
- Evaluating the diagnostic utility of the slide agglutination method for brucellosis in Saudi Arabia: A retrospective study at International Medical Center, Jeddah, Saudi Arabia.PLoS neglected tropical diseases · 2025Article
- Serological diagnosis of human brucellosis in Morocco and prospects for advanced diagnostic techniques.Iranian journal of microbiology · 2025Article
- Comparative Evaluation of Diagnostic Tests for Brucellosis in Humans and Animals: A Meta-Analytical Approach.Veterinary sciences · 2025Article
- Brucella-associated hemophagocytic syndrome: case report of a potentially life-threatening condition and literature review.Frontiers in immunology · 2025Review
- Unraveling brucellosis: advances in pathogenesis, diagnostic strategies, therapeutic innovations, and public health perspectives.Frontiers in medicine · 2025Review
- Diagnosis of brucellosis: Combining tests to improve performance.PLoS neglected tropical diseases · 2024Observational
- Seroepidemiology and associated risk factors of brucellosis in small ruminants of district Khanewal, Pakistan.Journal of advanced veterinary and animal research · 2024Article
- Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa.Pathogens (Basel, Switzerland) · 2024Article
- Seroprevalence and risk factor analysis of brucellosis among dairy farmers in Aligarh region, North India: creating awareness of a neglected disease.Access microbiology · 2024Article
- Molecular detection ofIranian journal of veterinary research · 2023Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis study aims to evaluate the efficacy of the standard agglutination test (SAT), the Brucellacapt test and enzyme-linked immunosorbent assay (ELISA) in clinical specimens collected from patients with suspected brucellosis.
methodsA prospective study was conducted from December 2020 to December 2021. Brucellosis was diagnosed on the basis of clinical evidence, and confirmed by isolation of Brucella or a four-fold rise in SAT titer. All samples were tested by the SAT, ELISA and the Brucellacapt test. Titers ≥1:100 were considered as SAT positive; ELISA was considered positive when an index greater than 11 was detected, while titers ≥1/160 indicated positivity on the Brucellacapt test. The specificity, sensitivity, and positive (PPVs) and negative predictive values (NPVs) of the three different methods were calculated.
resultsA total of 149 samples were collected from patients with suspected brucellosis. The sensitivities for the SAT, IgG, and IgM detection were 74.42%, 88.37% and 74.42%, respectively. The specificities were 95.24%, 93.65%, and 88.89%, respectively. The simultaneous measurement of IgG and IgM improved the sensitivity (98.84%) but reduced the specificity (84.13%) compared to each antibody test separately. The Brucellacapt test had excellent specificity (100%) and a high PPV (100%); however, the sensitivity and NPV were 88.37% and 86.30%, respectively. The combination of IgG detection by ELISA and the Brucellacapt test had excellent diagnostic performance, with 98.84% sensitivity and 93.65% specificity.
conclusionThis study showed that the simultaneous performance of IgG detection by ELISA and the Brucellacapt test has the potential to overcome the current limitations of detection.
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