ArticleeLife2023
Nitrogenase resurrection and the evolution of a singular enzymatic mechanism.
Article in eLife, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Evolution-guided engineering of an ancient nitrogenase interface enhances enzyme activity and stability.bioRxiv : the preprint server for biology · 2026Article
- Resurrected nitrogenases recapitulate canonical N-isotope biosignatures over two billion years.Nature communications · 2026Article
- Adaptation of Fe-S Cluster Assembly to Rising OResearch square · 2026Article
- Ecological constraints and evolutionary trade-offs shape nitrogen fixation across habitats.ISME communications · 2026Article
- Comparative Dynamics Enables Discovery of Embedded Bacterial Ferredoxin Domains in Large Redox Enzymes.Proteins · 2025Article
- A scaffold for quinone channeling between membrane and soluble bacterial oxidoreductases.Nature structural & molecular biology · 2025Article
- Article
- CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes.Structure (London, England : 1993) · 2025Article
- Nitrogen stable isotope fractionation by biological nitrogen fixation reveals cellular nitrogenase is diffusion limited.PNAS nexus · 2025Article
- Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotrophScience advances · 2024Article
- Ancient nitrogenases are ATP dependent.mBio · 2024Article
- Emergence of an Orphan Nitrogenase Protein Following Atmospheric Oxygenation.Molecular biology and evolution · 2024Article
- Article
- On the Shoulders of Giants-Reaching for Nitrogenase.Molecules (Basel, Switzerland) · 2023Review
- Regulatory response to a hybrid ancestral nitrogenase inMicrobiology spectrum · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The planetary biosphere is powered by a suite of key metabolic innovations that emerged early in the history of life. However, it is unknown whether life has always followed the same set of strategies for performing these critical tasks. Today, microbes access atmospheric sources of bioessential nitrogen through the activities of just one family of enzymes, nitrogenases. Here, we show that the only dinitrogen reduction mechanism known to date is an ancient feature conserved from nitrogenase ancestors. We designed a paleomolecular engineering approach wherein ancestral nitrogenase genes were phylogenetically reconstructed and inserted into the genome of the diazotrophic bacterial model,
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.