ArticlePlant physiology2023
Persulfidation of transcription factor MYB10 inhibits anthocyanin synthesis in red-skinned pear.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 42 citations in OpenAlex.
- Light-responsive PyNAC90 orchestrates anthocyanin biosynthesis through combinatorial transcriptional activation and COP1-mediated degradation in pear.Molecular horticulture · 2026Article
- Hydrogen sulfide-mediated persulfidation of ERF106 controls pear fruit aroma biosynthesis via disrupting the ERF106-MYB21-FAD8 complex.The Plant journal : for cell and molecular biology · 2026Article
- Hydrogen sulfide primes drought tolerance in plants by metabolic control of DNA methylation.iScience · 2026Article
- From odor to order: unveiling the crucial role of hydrogen sulfide in plant life.Horticulture research · 2026Article
- Alfalfa Transcription Factor MsNAC2a Orchestrates the Homeostasis of Salt Stress Responses via Reactive Oxygen Species Accumulation and Hydrogen Sulphide Depletion.Plant biotechnology journal · 2025Article
- Functional interactions among H2O2, NO, H2S, and melatonin in the physiology, metabolism, and quality of horticultural Solanaceae.Journal of experimental botany · 2025Review
- Nitrogen signaling factor triggers a respiration-like gene expression program in fission yeast.The EMBO journal · 2024Article
- An 'activator-repressor' loop controls the anthocyanin biosynthesis in red-skinned pear.Molecular horticulture · 2024Article
- Persulfidome of Sweet Pepper Fruits during Ripening: The Case Study of Leucine Aminopeptidase That Is Positively Modulated by HAntioxidants (Basel, Switzerland) · 2024Article
- Integrated metabolomic and transcriptomic dynamic profiles of endopleura coloration during fruit maturation in three walnut cultivars.BMC plant biology · 2024Article
- Hydrogen Sulfide in the Oxidative Stress Response of Plants: Crosstalk with Reactive Oxygen Species.International journal of molecular sciences · 2024Review
- Focus on fruit crops.Plant physiology · 2023Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule that delays color change during fruit ripening. Whether H2S affects anthocyanin biosynthesis in red-skinned pears (Pyrus L.) remains unclear. Here, we found that H2S substantially inhibits anthocyanin accumulation in red-skinned pears and the expression of several genes encoding transcription factors is affected in response to H2S signaling. For example, PyMYB10 and PyMYB73 were down-regulated, whereas PyMYB114 and PyMYB6 were up-regulated. Bioinformatics analysis showed that PyMYB73 and PyMYB6, each containing an EAR motif, may negatively regulate anthocyanin accumulation. Transient expression analysis showed that PyMYB73 substantially promotes anthocyanin biosynthesis by co-transforming with PyMYB10/PyMYB114 + PybHLH3; however, PyMYB6 inhibited anthocyanin biosynthesis in strawberry (Fragaria vesca) receptacles and pear fruits, and PyMYB73 interacted with PyMYB10 and PyMYB6 but not PyMYB114 or PybHLH3. Further investigation showed that Cys194 and Cys218 of PyMYB10 were modified by persulfidation and that PyMYB10Cys218Ala substantially increased anthocyanin accumulation by a transient transformation system. Co-transformation of PyMYB10Cys218Ala + PyMYB73/PyMYB6 also promoted anthocyanin accumulation in pear fruits. Yeast two-hybrid assays showed that the mutation of PyMYB10 did not affect the interaction between PyMYB10 and PyMYB73, but it inhibited interaction with PyMYB6. Moreover, H2S weakened the interaction between PyMYB10 and PyMYB73 but enhanced the interaction with PyMYB6. Thus, we provided a model in which PyMYB10 undergoes persulfidation at Cys218, enhancing the interaction with PyMYB6 and reducing the interaction with PyMYB73. These subsequently results in lower expression of the anthocyanin biosynthesis-related genes Pyrus dihydroflavonol 4-reductase (PyDFR), Pyrus anthocyanidin synthase (PyANS), Pyrus UDP-glucose: flavonoid 3-glucosyl transferase (PyUFGT) and Pyrus glutathione S-transferase (PyGST), thereby inhibiting anthocyanin accumulation in red-skinned pears. Our findings provided a molecular mechanism for H2S-mediated anthocyanin biosynthesis in red-skinned pears.
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