Evidence map›Paper›PMID 36795366›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

A Unique Multiplex ELISA to Profile Growth Factors and Cytokines in Cerebrospinal Fluid.

Charitha Madiraju, Amani Sastry, Martha Oppong, Jacob Karp, Maryla Krajewska, Stan Krajewski, Barbara Tomik, Andrzej Szczudlik, Robert S Matson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Charitha MadirajuMarshall B. Ketchum University, Fullerton, CA, USA. cmadiraju@ketchum.edu.
Amani SastryNoorda College of Osteopathic Medicine, Provo, UT, USA.
Martha OppongMarshall B. Ketchum University, Fullerton, CA, USA.
Jacob KarpMarshall B. Ketchum University, Fullerton, CA, USA.
Maryla KrajewskaCellestan Immunoquant (CIQ), Inc, Oceanside, CA, USA.
Stan KrajewskiCellestan Immunoquant (CIQ), Inc, Oceanside, CA, USA.
Barbara TomikDepartment of Neurology, Jagiellonian University Medical College, Krakow, Poland.
Andrzej SzczudlikDepartment of Neurology, Jagiellonian University Medical College, Krakow, Poland.
Robert S MatsonQuantiScientifics, LLC, Orange, CA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multiplex arrays designed for enzyme-linked immunosorbent assays (ELISAs) are robust and cost-effective for profiling biomarkers. Identification of relevant biomarkers in biological matrices or fluids helps in the understanding of disease pathogenesis. Here, we describe a sandwich ELISA-based multiplex assay to assess growth factor and cytokine levels in cerebrospinal fluid (CSF) samples derived from multiple sclerosis patients, amyotrophic lateral sclerosis patients, and control subjects without any neurological disorder. Results indicate that multiplex assay designed for the sandwich ELISA method is a unique, robust, and cost-effective method for profiling growth factors and cytokines present in CSF samples.

Indexed as

Amyotrophic Lateral SclerosisCytokinesBiomarkersEnzyme-Linked Immunosorbent AssayHumansIntercellular Signaling Peptides and ProteinsBiomarkersCytokinesIntercellular Signaling Peptides and ProteinsAmyotrophic lateral sclerosis (ALS)BiomarkersCerebrospinal fluid samples (CSFs)CytokinesGrowth factorsMultiple sclerosis (MS)Multiplex arraySandwich ELISA

Identifiers

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.