Evidence map›Paper›PMID 36795297›Full record

ArticleAngiogenesis2023

Modeling angiogenesis in the human brain in a tissue-engineered post-capillary venule.

Nan Zhao, Sarah Kulkarni, Sophia Zhang, Raleigh M Linville, Tracy D Chung, Zhaobin Guo, John J Jamieson, Danielle Norman, Lily Liang, Alexander F Pessell and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angiogenesis, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Nan ZhaoInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Sarah KulkarniDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Sophia ZhangDepartment of Biology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Raleigh M LinvilleInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Tracy D ChungInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Zhaobin GuoInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
John J JamiesonInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Danielle NormanDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Lily LiangInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Alexander F PessellInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Peter SearsonInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA. searson@jhu.edu.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

BIOMEDICAL ENGINEERING TRAINING PROGRAMT32GM007057 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI KANOLD, PATRICK O, KARCHIN, RACHEL · 1985 to 2023
$10.4M
Mechanisms of cerebrovascular barrier dysfunction caused by APP and PSEN1 mutations and amyloid beta exposureR01NS106008 · NINDS · JOHNS HOPKINS UNIVERSITY · PI SEARSON, PETER C · 2019 to 2023
$1.9M
Reverse engineering zonation-specific and age-specific iPSC-derived cerebrovascular models based on transcriptomic profiling of the human brainR61HL154252 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HEIMAN, MYRIAM, SEARSON, PETER C · 2021 to 2022
$1.6M
Elucidating the role of pericytes in angiogenesis in the brain using a tissue-engineered microvessel modelR21NS131831 · NINDS · JOHNS HOPKINS UNIVERSITY · PI SEARSON, PETER C · 2023 to 2024
$450k
NHLBI NIH HHS R61 HL154252NIGMS NIH HHS T32 GM007057NINDS NIH HHS R01 NS106008NINDS NIH HHS R21 NS131831
6 · The paper itself

Abstract

Angiogenesis plays an essential role in embryonic development, organ remodeling, wound healing, and is also associated with many human diseases. The process of angiogenesis in the brain during development is well characterized in animal models, but little is known about the process in the mature brain. Here, we use a tissue-engineered post-capillary venule (PCV) model incorporating stem cell derived induced brain microvascular endothelial-like cells (iBMECs) and pericyte-like cells (iPCs) to visualize the dynamics of angiogenesis. We compare angiogenesis under two conditions: in response to perfusion of growth factors and in the presence of an external concentration gradient. We show that both iBMECs and iPCs can serve as tip cells leading angiogenic sprouts. More importantly, the growth rate for iPC-led sprouts is about twofold higher than for iBMEC-led sprouts. Under a concentration gradient, angiogenic sprouts show a small directional bias toward the high growth factor concentration. Overall, pericytes exhibited a broad range of behavior, including maintaining quiescence, co-migrating with endothelial cells in sprouts, or leading sprout growth as tip cells.

Indexed as

Endothelial CellsNeovascularization, PhysiologicAnimalsBrainCapillariesHumansVenulesAngiogenesisAngiogenic sproutBlood-brain barrierPericytesTip cellTissue engineering

Identifiers

PMID36795297
PMCPMC10789151
OpenAlexW4321002268

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.