ArticleNucleic acids research2023
Structure, substrate binding and activity of a unique AAA+ protein: the BrxL phage restriction factor.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.Nucleic acids research · 2026Article
- Competing forms of protein-protein association and DNA binding exhibited by BrxC from the BREX phage restriction system.bioRxiv : the preprint server for biology · 2026Article
- Structural basis of Retron-Eco8-mediated antiphage defense.Nucleic acids research · 2026Article
- Expanding the landscape of BREX diversity: uncovering multi-layered functional frameworks and identification of novel BREX-related defense systems.Nucleic acids research · 2026Article
- Bacterial defense systems: Mechanisms, homology to eukaryotic immune systems, and applications.Zoological research · 2026Review
- Modified DNA substrate selectivity by GmrSD-family Type IV restriction enzyme BrxU.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- PglZ from Type I BREX phage defence systems is a metal-dependent nuclease that forms a sub-complex with BrxB.Nucleic acids research · 2025Article
- Unveiling the multifaceted domain polymorphism of the Menshen antiphage system.Nucleic acids research · 2025Article
- Molecular basis of foreign DNA recognition by BREX anti-phage immunity system.Nature communications · 2025Article
- The vibriophage-encoded inhibitor OrbA abrogates BREX-mediated defense through the ATPase BrxC.Journal of bacteriology · 2024Article
- Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence.Nature communications · 2024Article
- Ocr-mediated suppression of BrxX unveils a phage counter-defense mechanism.Nucleic acids research · 2024Article
- Diversity and phage sensitivity to phages of porcine enterotoxigenicApplied and environmental microbiology · 2024Article
- Bacteriophage protein Dap1 regulates evasion of antiphage immunity and Pseudomonas aeruginosa virulence impacting phage therapy in mice.Nature microbiology · 2024Article
- Episymbiotic Saccharibacteria TM7x modulates the susceptibility of its host bacteria to phage infection and promotes their coexistence.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mutations in the C-terminal region of the bacteriophage exclusion protein PglX can selectively inactivate restriction inJournal of bacteriology · 2023Article
- Diverse Durham collection phages demonstrate complex BREX defense responses.Applied and environmental microbiology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Bacteriophage exclusion ('BREX') systems are multi-protein complexes encoded by a variety of bacteria and archaea that restrict phage by an unknown mechanism. One BREX factor, termed BrxL, has been noted to display sequence similarity to various AAA+ protein factors including Lon protease. In this study we describe multiple CryoEM structures of BrxL that demonstrate it to be a chambered, ATP-dependent DNA binding protein. The largest BrxL assemblage corresponds to a dimer of heptamers in the absence of bound DNA, versus a dimer of hexamers when DNA is bound in its central pore. The protein displays DNA-dependent ATPase activity, and ATP binding promotes assembly of the complex on DNA. Point mutations within several regions of the protein-DNA complex alter one or more in vitro behaviors and activities, including ATPase activity and ATP-dependent association with DNA. However, only the disruption of the ATPase active site fully eliminates phage restriction, indicating that other mutations can still complement BrxL function within the context of an otherwise intact BREX system. BrxL displays significant structural homology to MCM subunits (the replicative helicase in archaea and eukaryotes), implying that it and other BREX factors may collaborate to disrupt initiation of phage DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.