Evidence map›Paper›PMID 36794663›Full record

ArticleRenal failure2023

Bone marrow mesenchymal stem cell-derived exosomal miR-30e-5p ameliorates high-glucose induced renal proximal tubular cell pyroptosis by inhibiting ELAVL1.

Jia Lv, Ya-Ning Hao, Xiao-Pei Wang, Wan-Hong Lu, Li-Yi Xie, Dan Niu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Renal failure, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 41 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jia LvDepartment of Nephrology, College of Medicine, Nephrotic Hospital, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, PR China.
Ya-Ning HaoDepartment of Nephrology, College of Medicine, Nephrotic Hospital, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, PR China.
Xiao-Pei WangDepartment of Nephrology, College of Medicine, Nephrotic Hospital, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, PR China.
Wan-Hong LuDepartment of Nephrology, College of Medicine, Nephrotic Hospital, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, PR China.
Li-Yi XieDepartment of Nephrology, College of Medicine, Nephrotic Hospital, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, PR China.
Dan NiuDepartment of Nephrology, College of Medicine, Nephrotic Hospital, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, PR China.
First Affiliated Hospital of Xi'an Jiaotong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe rapid increase in the prevalence of diabetes has resulted in more cases of diabetic kidney disease (DKD). Treatment with bone marrow mesenchymal stem cells (BMSCs) may represent an alternative strategy to manage DKD.

methodsHK-2 cells were treated with 30 mM high glucose (HG). Bone marrow MSC-derived exosomes (BMSC-exos) were isolated and internalized into HK-2 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and lactate dehydrogenase (LDH) assays were used to measure viability and cytotoxicity. The secretion of IL-1β and IL-18 was measured by ELISA. Pyroptosis was assessed by flow cytometry. Quantitative RT-PCR was used to measure the levels of miR-30e-5p, ELAV like RNA binding protein 1 (ELAVL1), IL-1β, and IL-18. The expression of ELAVL1 and pyroptosis-associated cytokine proteins was determined by western blot analysis. A dual-luciferase reporter gene assay was conducted to confirm the relationship between miR-30e-5p and ELAVL1.

resultsBMSC-exos decreased LDH, IL-1β, and IL-18 secretion and inhibited the expression of the pyroptosis-related factors (IL-1β, caspase-1, GSDMD-N, and NLRP3) in HG-induced HK-2 cells. Moreover, miR-30e-5p depletion derived from BMSC-exos promoted HK-2 cell pyroptosis. Besides, miR-30e-5p over-expression or ELVAL1 knockdown could directly inhibit pyroptosis. ELAVL1 was a target of miR-30e-5p and knocking down ELAVL1 reversed the effect of miR-30e-5p inhibition in BMSC-exos-treated HK-2 cells.

conclusionsBMSC-derived exosomal miR-30e-5p inhibits caspase-1-mediated pyroptosis by targeting ELAVL1 in HG-induced HK-2 cells, which might provide a new strategy for treating DKD.

Indexed as

ELAV-Like Protein 1Mesenchymal Stem CellsMicroRNAsCaspasesCell LineExosomesGlucoseHumansInterleukin-18Kidney Tubules, ProximalPyroptosisCaspasesELAVL1 protein, humanELAV-Like Protein 1GlucoseInterleukin-18MicroRNAsMIRN30e microRNA, humanbone marrow mesenchymal stem cellsdiabetic kidney diseaseexosomemiR-30e-5pPyroptosis

Identifiers

PMID36794663
PMCPMC9937013
OpenAlexW4321003532

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.