ReviewJournal of molecular evolution2023
Revisiting the Design of the Long-Term Evolution Experiment with Escherichia coli.
Review in Journal of molecular evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Simulating the host niche: balancing complexity and control in the experimental evolution of antibiotic resistance and pathoadaptation.Microbiology (Reading, England) · 2026Review
- Additive effects of environmental and demographic variation shape the repeatability of evolution across replicated experiments.Evolution letters · 2026Article
- Identifiability, Sensitivity, and Genetic Algorithms in Bacterial Biofilm Selection Models.Bulletin of mathematical biology · 2026Article
- Demand-only energetics at 120 ATP per glucose: A reply to Lynch.Biochimica et biophysica acta. Bioenergetics · 2026Article
- Selection for Molecularly Complementary Modules (MCMs) Drives the Origins and Evolution of Pleiofunctional, Epistatic Interactomes (PEIs).Life (Basel, Switzerland) · 2026Review
- Emergence of supercoiling-mediated regulatory networks through the evolution of bacterial chromosome organization.PLoS computational biology · 2025Article
- Creating a consensus genome assembly ofNAR genomics and bioinformatics · 2025Article
- Frequency-dependent fitness effects are ubiquitous.bioRxiv : the preprint server for biology · 2025Article
- Paradox of the Sub-Plankton: Plausible Mechanisms and Open Problems Underlying Strain-Level Diversity in Microbial Communities.Environmental microbiology · 2025Review
- Review
- Rhizosphere domestication enhances root colonization and plant growth promotion performance ofFrontiers in microbiology · 2025Article
- Quantifying Competitive Fitness in Yeast with High-Throughput Fluorescence Microscopy Imaging.Current protocols · 2025Article
- Trace elements increase replicability of microbial growth.Open biology · 2025Article
- Review
- Effects of periodic bottlenecks on the dynamics of adaptive evolution in microbial populations.Microbiology (Reading, England) · 2024Article
- A new concept of biocatalytic synthesis of acrylic monomers for obtaining water-soluble acrylic heteropolymers.Metabolic engineering communications · 2024Article
- Genomics for monitoring and understanding species responses to global climate change.Nature reviews. Genetics · 2024Review
- Assessing the predictability of fungicide resistance evolution through in vitro selection.Journal of plant diseases and protection : scientific journal of the German Phytomedical Society (DPG) · 2024Article
- Evolution of a cross-feeding interaction following a key innovation in a long-term evolution experiment withMicrobiology (Reading, England) · 2023Article
- Dynamics ofInternational journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
The long-term evolution experiment (LTEE) with Escherichia coli began in 1988 and it continues to this day, with its 12 populations having recently reached 75,000 generations of evolution in a simple, well-controlled environment. The LTEE was designed to explore open-ended questions about the dynamics and repeatability of phenotypic and genetic evolution. Here I discuss various aspects of the LTEE's experimental design that have enabled its stability and success, including the choices of the culture regime, growth medium, ancestral strain, and statistical replication. I also discuss some of the challenges associated with a long-running project, such as handling procedural errors (e.g., cross-contamination) and managing the expanding collection of frozen samples. The simplicity of the experimental design and procedures have supported the long-term stability of the LTEE. That stability-along with the inherent creativity of the evolutionary process and the emergence of new genomic technologies-provides a platform that has allowed talented students and collaborators to pose questions, collect data, and make discoveries that go far beyond anything I could have imagined at the start of the LTEE.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.