ArticleDevelopment (Cambridge, England)2023
Production and characterization of monoclonal antibodies to Xenopus proteins.
Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 12 citations in OpenAlex.
- Identification of a novel N-terminal linear B-cell epitope on the FIPV nucleocapsid protein by a monoclonal antibody.BMC veterinary research · 2026Article
- Planar polarization of endogenous ADIP during Xenopus neurulation.Biology open · 2026Article
- Adam13 interacts with large protein complexes to regulate histone modification and gene expression.Frontiers in cell and developmental biology · 2026Article
- Spinal Cord Regeneration in Xenopus.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A Dapl1Science advances · 2025Article
- Mechanical cues polarize ADIP protein complexes to control vertebrate morphogenesis and wound healing.Current biology : CB · 2025Article
- Cardiomyocyte proliferation and heart regeneration in adult Xenopus tropicalis evidenced by a transgenic reporter line.NPJ Regenerative medicine · 2024Article
- SoxB1 transcription factors are essential for initiating and maintaining neural plate border gene expression.Development (Cambridge, England) · 2024Article
- Essential role of MgCell reports · 2023Article
- ADAM11 a novel regulator of Wnt and BMP4 signaling in neural crest and cancer.Frontiers in cell and developmental biology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple Xenopus proteins and their validation in various experimental approaches. These novel antibodies are now available to the research community through the Developmental Study Hybridoma Bank (DSHB).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.