Evidence map›Paper›PMID 36789432›Full record

ArticleResearch square2023

Intravenous functional gene transfer throughout the brain of non-human primates using AAV.

Miguel R Chuapoco, Nicholas C Flytzanis, Nick Goeden, J Christopher Octeau, Kristina M Roxas, Ken Y Chan, Jon Scherrer, Janet Winchester, Roy J Blackburn, Lillian J Campos and 22 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

32 authors at 6 institutions in 1 country.

Miguel R ChuapocoDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Nicholas C FlytzanisDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Nick GoedenDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
J Christopher OcteauCapsida Biotherapeutics, Thousand Oaks, CA 91320, USA.
Kristina M RoxasCapsida Biotherapeutics, Thousand Oaks, CA 91320, USA.
Ken Y ChanDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Jon ScherrerCapsida Biotherapeutics, Thousand Oaks, CA 91320, USA.
Janet WinchesterCapsida Biotherapeutics, Thousand Oaks, CA 91320, USA.
Roy J BlackburnCapsida Biotherapeutics, Thousand Oaks, CA 91320, USA.
Lillian J CamposDepartment of Psychology and the California National Primate Research Center, University of California-Davis, Davis, CA 95616, USA.
Kwun Nok Mimi ManDepartment of Psychology and the California National Primate Research Center, University of California-Davis, Davis, CA 95616, USA.
Junqing SunDepartment of Psychology and the California National Primate Research Center, University of California-Davis, Davis, CA 95616, USA.
Xinhong ChenDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.ORCID 0000-0003-0408-0813
Arthur LefevreCortical Systems and Behavior Laboratory, University of California-San Diego, La Jolla, CA 92039, USA.
Vikram Pal SinghCortical Systems and Behavior Laboratory, University of California-San Diego, La Jolla, CA 92039, USA.
Cynthia M ArokiarajDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.ORCID 0000-0003-3201-9868
Timothy F ShayaDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.ORCID 0000-0001-6591-3271
Julia VendemiattiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Min J JangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
John MichAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Yeme BishawAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Bryan GoreAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Victoria OmsteadAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Naz TaskinAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Natalie WeedAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Jonathan TingAllen Institute for Brain Science, Seattle, WA, 98109, USA.
Cory T MillerCortical Systems and Behavior Laboratory, University of California-San Diego, La Jolla, CA 92039, USA.
Benjamin E DevermanDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
James PickelCortical Systems and Behavior Laboratory, University of California-San Diego, La Jolla, CA 92039, USA.
Lin TianDepartment of Psychology and the California National Primate Research Center, University of California-Davis, Davis, CA 95616, USA.ORCID 0000-0001-7012-6929
Andrew S FoxDepartment of Psychology and the California National Primate Research Center, University of California-Davis, Davis, CA 95616, USA.
Viviana GradinaruDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.ORCID 0000-0001-5868-348X
Allen Institute for Brain Science · USCalifornia Institute of Technology · USNanotherapeutics (United States) · USUniversity of California, Davis · USUniversity of California San Diego · USBroad Institute · US

Funding

National Institute on Aging (NIA) ColonyP51OD011107 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Simon J. Atkinson · 2012 to 2026
$191.5M
Engineered AAV Identification, Validation, and Dissemination Pipeline for Brain Cell Type-Specific Manipulation Across SpeciesUF1MH128336 · NIMH · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI FOX, ANDREW S, GRADINARU, VIVIANA · 2021 to 2021
$6.1M
NIH HHS P51 OD011107NIMH NIH HHS UF1 MH128336
6 · The paper itself

Abstract

Adeno-associated viruses (AAVs) promise robust gene delivery to the brain through non-invasive, intravenous delivery. However, unlike in rodents, few neurotropic AAVs efficiently cross the blood-brain barrier in non-human primates (NHPs). Here we describe AAV.CAP-Mac, an engineered variant identified by screening in adult marmosets and newborn macaques with improved efficiency in the brain of multiple NHP species: marmoset, rhesus macaque, and green monkey. CAP-Mac is neuron-biased in infant Old World primates, exhibits broad tropism in adult rhesus macaques, and is vasculature-biased in adult marmosets. We demonstrate applications of a single, intravenous dose of CAP-Mac to deliver (1) functional GCaMP for ex vivo calcium imaging across multiple brain areas, and (2) a cocktail of fluorescent reporters for Brainbow-like labeling throughout the macaque brain, circumventing the need for germline manipulations in Old World primates. Given its capabilities for systemic gene transfer in NHPs, CAP-Mac promises to help unlock non-invasive access to the brain.

Identifiers

PMID36789432
PMCPMC9928057
OpenAlexW4315767697

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.