Evidence map›Paper›PMID 36789332›Full record

ArticleEcology and evolution2023

Parasite detection and quantification in avian blood is dependent on storage medium and duration.

Joshua G Lynton-Jenkins, Alexis S Chaine, Andrew F Russell, Camille Bonneaud

Open access · goldAbstract read
In one paragraph

Article in Ecology and evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.2field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Joshua G Lynton-JenkinsCentre for Ecology and Conservation University of Exeter Penryn UK.ORCID https://orcid.org/0000-0003-1657-7843
Alexis S ChaineStation for Theoretical and Experimental Ecology CNRS Moulis France.ORCID https://orcid.org/0000-0003-3346-551X
Andrew F RussellCentre for Ecology and Conservation University of Exeter Penryn UK.ORCID https://orcid.org/0000-0002-1299-8555
Camille BonneaudCentre for Ecology and Conservation University of Exeter Penryn UK.ORCID https://orcid.org/0000-0003-2248-3288
Centre National de la Recherche Scientifique · FRUniversity of Exeter · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studies of parasites in wild animal populations often rely on molecular methods to both detect and quantify infections. However, method accuracy is likely to be influenced by the sampling approach taken prior to nucleic acid extraction. Avian Haemosporidia are studied primarily through the screening of host blood, and a range of storage mediums are available for the short- to long-term preservation of samples. Previous research has suggested that storage medium choice may impact the accuracy of PCR-based parasite detection, however, this relationship has never been explicitly tested and may be exacerbated by the duration of sample storage. These considerations could also be especially critical for sensitive molecular methods used to quantify infection (qPCR). To test the effect of storage medium and duration on

Indexed as

avian malariaDNA extractionPCRplasmodiumqPCRQueen's lysis buffer

Identifiers

PMID36789332
PMCPMC9911630
OpenAlexW4319984364

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.