Evidence map›Paper›PMID 36789083›Full record

ArticleBio-protocol2023

Staining and Scanning Protocol for Micro-Computed Tomography to Observe the Morphology of Soft Tissues in Ambrosia Beetles.

Ellie J Spahr, Sarah L McLaughlin, Alyssa M Tichinel, Matt T Kasson, Teiya Kijimoto

Open access · diamondAbstract read
In one paragraph

Article in Bio-protocol, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ellie J SpahrWest Virginia University Division of Plant and Soil Sciences, 1194 Evansdale Drive Morgantown, WV, USA.
Sarah L McLaughlinWest Virginia University Animal Models and Imaging Facility, Health Sciences Center, Morgantown, WV, USA.
Alyssa M TichinelWest Virginia University Animal Models and Imaging Facility, Health Sciences Center, Morgantown, WV, USA.
Matt T KassonWest Virginia University Division of Plant and Soil Sciences, 1194 Evansdale Drive Morgantown, WV, USA.
Teiya KijimotoWest Virginia University Division of Plant and Soil Sciences, 1194 Evansdale Drive Morgantown, WV, USA.
West Virginia University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advances in imaging technology offer new opportunities in developmental biology. To observe the development of internal structures, microtome cross-sectioning followed by H&E staining on glass slides is a common procedure; however, this technique can be destructive, and artifacts can be introduced during the process. In this protocol, we describe a less invasive procedure with which we can stain insect samples and obtain reconstructed three-dimensional images using micro-computed tomography, or micro-CT (µCT). Specifically, we utilize the fungus-farming ambrosia beetle species

Indexed as

Ambrosia beetleEuwallacea validusMicro-computed tomographyMycangiaSoft tissueSymbiosis

Identifiers

PMID36789083
PMCPMC9901460
OpenAlexW4313588765

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.