ArticleScientific reports2023
Diagnosis of Fusarium oxysporum f. sp. ciceris causing Fusarium wilt of chickpea using loop-mediated isothermal amplification (LAMP) and conventional end-point PCR.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Computational studies deciphered the role of key genes and associated networks regulating the defense mechanism in chickpea underPlant signaling & behavior · 2026Article
- Towards a Phylogenomic Framework for theInternational journal of molecular sciences · 2026Article
- Involvement ofJournal of fungi (Basel, Switzerland) · 2026Article
- Genetic and Pathogenic Differentiation ofJournal of fungi (Basel, Switzerland) · 2026Article
- Rapid and specific detection of Peronospora belbahrii in basil (Ocimum basilicum L.) using a LAMP assay.Scientific reports · 2026Article
- Antifungal activities of Rosmarinus extracts against Fusarium oxysporum, the pathogenic fungus of Anoectochilus stem rot.BMC plant biology · 2025Article
- Phenotypic diversity, symbiotic effectiveness and plant growth promoting characteristics of rhizobia nodulating chickpea (Cicer arietinum L.) from central and south gondar zones, Ethiopia.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Loop mediated isothermal amplification (LAMP) as a rapid and portable diagnostic tool for the detection of pea root rot pathogens.Scientific reports · 2025Article
- Development and comparative evaluation of LAMP, nested PCR and Real-time PCR assays for detecting Fusarium tricinctum, a fungal pathogen of Zanthoxylum bungeanum.BMC microbiology · 2025Article
- Comparative Antagonistic Activities of Endolichenic Fungi Isolated from the Fruticose LichensJournal of fungi (Basel, Switzerland) · 2025Article
- The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars.BMC genomics · 2025Article
- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
- Characterization of the High-Quality Genome Sequence and Virulence Factors ofJournal of fungi (Basel, Switzerland) · 2024Article
- Evaluation of biocontrol efficacy of rhizosphere dwelling bacteria for management of Fusarium wilt and Botrytis gray mold of chickpea.BMC genomic data · 2024Article
- Advances in the detection technology of vegetable soil borne fungi and bacteria.Frontiers in microbiology · 2024Review
- Breeding and Genomic Approaches towards Development of Fusarium Wilt Resistance in Chickpea.Life (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium oxysporum (Fo) is ubiquitous in soil and forms a species complex of pathogenic and putatively non-pathogenic strains. Pathogenic strains cause disease in over 150 plant species. Fusarium oxysporum f. sp. ciceris (Foc) is a major fungal pathogen causing Fusarium wilt in chickpeas (Cicer arietinum). In some countries such as Australia, Foc is a high-priority pest of biosecurity concern. Specific, sensitive, robust and rapid diagnostic assays are essential for effective disease management on the farm and serve as an effective biosecurity control measure. We developed and validated a novel and highly specific PCR and a LAMP assay for detecting the Indian Foc race 1 based on a putative effector gene uniquely present in its genome. These assays were assessed against 39 Fo formae speciales and found to be specific, only amplifying the target species, in a portable real-time fluorometer (Genie III) and qPCR machine in under 13 min with an anneal derivative temperature ranging from 87.7 to 88.3 °C. The LAMP assay is sensitive to low levels of target DNA (> 0.009 ng/µl). The expected PCR product size is 143 bp. The LAMP assay developed in this study was simple, fast, sensitive and specific and could be explored for other Foc races due to the uniqueness of this marker to the Foc genome.
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