Evidence map›Paper›PMID 36787336›Full record

ArticlePloS one2023

Proteomic analysis of peripheral blood mononuclear cells isolated from patients with pulmonary tuberculosis: A pilot study from Zanzibar, Tanzania.

Ahmed Barakat, Even Birkeland, Melissa D Jørstad, Magalie El Hajj, Msafiri Marijani, Anne Døskeland, Olav Mjaavatten, Frode S Berven, Tehmina Mustafa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Ahmed BarakatCentre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
Even BirkelandProteomics Unit at University of Bergen (PROBE), Department of Biomedicine, University of Bergen, Bergen, Norway.
Melissa D JørstadCentre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
Magalie El HajjCentre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
Msafiri MarijaniDepartment of Diagnostic Services, Mnazi Mmoja Hospital, Zanzibar, The United Republic of Tanzania.
Anne DøskelandProteomics Unit at University of Bergen (PROBE), Department of Biomedicine, University of Bergen, Bergen, Norway.
Olav MjaavattenProteomics Unit at University of Bergen (PROBE), Department of Biomedicine, University of Bergen, Bergen, Norway.
Frode S BervenProteomics Unit at University of Bergen (PROBE), Department of Biomedicine, University of Bergen, Bergen, Norway.
Tehmina MustafaCentre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.ORCID 0000-0001-9822-1191
University of Bergen · NOHaukeland University Hospital · NOZanzibar University · TZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed at exploring the proteomic profile of PBMCs to predict treatment response in pulmonary tuberculosis (PTB). This was a pilot study conducted among 8 adult patients from Zanzibar, Tanzania with confirmed PTB. Blood samples were collected at baseline, at 2 months of treatment, and at the end of treatment at 6 months. Proteins were extracted from PBMCs and analyzed using LC-MS/MS based label free quantitative proteomics. Overall, 3,530 proteins were quantified across the samples, and 12 differentially expressed proteins were identified at both 2 months of treatment and at treatment completion, which were involved in cellular and metabolic processes, as well as binding and catalytic activity. Seven were downregulated proteins (HSPA1B/HSPA1A, HSPH1, HSP90AA1, lipopolysaccharide-binding protein, complement component 9, calcyclin-binding protein, and protein transport protein Sec31A), and 5 proteins were upregulated (SEC14 domain and spectrin repeat-containing protein 1, leucine-rich repeat-containing 8 VRAC subunit D, homogentisate 1,2-dioxygenase, NEDD8-activating enzyme E1 regulatory subunit, and N-acetylserotonin O-methyltransferase-like protein). The results showed that proteome analysis of PBMCs can be used as a novel technique to identify protein abundance change with anti-tuberculosis treatment. The novel proteins elucidated in this work may provide new insights for understanding PTB pathogenesis, treatment, and prognosis.

Indexed as

Leukocytes, MononuclearTuberculosis, PulmonaryAdultChromatography, LiquidHumansPilot ProjectsProteomeProteomicsTandem Mass SpectrometryTanzaniaProteome

Identifiers

PMID36787336
PMCPMC9928017
OpenAlexW4320709791

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.