Evidence map›Paper›PMID 36787323›Full record

ArticlePloS one2023

Patients with abdominal aortic aneurysms have reduced levels of microRNA 122-5p in circulating exosomes.

Jose L Lopez, Joel L Ramirez, Tuan Anh Phu, Phat Duong, Laura Bouchareychas, Christina R Kuhrau, Pei-Yu Lin, Walter L Eckalbar, Andrea J Barczak, Joshua D Rudolph and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 4 citations in OpenAlex.

  1. Observational
  2. Review
  3. Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Jose L LopezDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.ORCID 0000-0001-8073-4124
Joel L RamirezDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Tuan Anh PhuDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Phat DuongDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Laura BouchareychasDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Christina R KuhrauDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.ORCID 0000-0002-0709-0497
Pei-Yu LinDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Walter L EckalbarDivision of Pulmonary and Critical Care Medicine, Department of Medicine, UCSF CoLabs, University of California San Francisco, San Francisco, California, United States of America.
Andrea J BarczakDivision of Pulmonary and Critical Care Medicine, Department of Medicine, UCSF CoLabs, University of California San Francisco, San Francisco, California, United States of America.
Joshua D RudolphDivision of Pulmonary and Critical Care Medicine, Department of Medicine, UCSF CoLabs, University of California San Francisco, San Francisco, California, United States of America.
Lenka MaliskovaDivision of Pulmonary and Critical Care Medicine, Department of Medicine, UCSF CoLabs, University of California San Francisco, San Francisco, California, United States of America.
Michael S ConteDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Shant M VartanianDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Robert L RaffaiDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
Adam Z OskowitzDivision of Vascular and Endovascular Surgery, Department of Surgery, University of California San Francisco, San Francisco, California, United States of America.
University of California, San Francisco · USSan Francisco VA Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThere are currently no specific biomarkers to identify patients with abdominal aortic aneurysms (AAAs). Circulating exosomes contain microRNAs (miRNA) that are potential biomarkers for the presence of disease. This study aimed to characterize the exosomal miRNA expression profile of patients with AAAs in order to identify novel biomarkers of disease.

methodsPatients undergoing duplex ultrasound (US) or computed tomography (CT) for screening or surveillance of an AAA were screened to participate in the study. Cases with AAA were defined as having a max aortic diameter >3 cm. Circulating plasma exosomes were isolated using Cushioned-Density Gradient Ultracentrifugation and total RNA was extracted. Next Generation Sequencing was performed on the Illumina HiSeq4000 SE50. Differential miRNA expression analysis was performed using DESeq2 software with a Benjamini-Hochberg correction. MicroRNA expression profiles were validated by Quantitative Real-Time PCR.

resultsA total of 109 patients were screened to participate in the study. Eleven patients with AAA and 15 non-aneurysmal controls met study criteria and were enrolled. Ultrasound measured aortic diameter was significantly larger in the AAA group (mean maximum diameter 4.3 vs 2.0 cm, P = 6.45x10-6). More AAA patients had coronary artery disease (5/11 vs 1/15, P = 0.05) as compared to controls, but the groups did not differ significantly in the rates of peripheral arterial disease and chronic obstructive pulmonary disease. A total of 40 miRNAs were differentially expressed (P<0.05). Of these, 18 miRNAs were downregulated and 22 were upregulated in the AAA group compared to controls. After false discovery rate (FDR) adjustment, only miR-122-5p was expressed at significantly different levels in the AAA group compared to controls (fold change = 5.03 controls vs AAA; raw P = 1.8x10-5; FDR P = 0.02).

conclusionPlasma exosomes from AAA patients have significantly reduced levels of miRNA-122-5p compared to controls. This is a novel exosome-associated miRNA that warrants further investigation to determine its use as a diagnostic biomarker and potential implications in AAA pathogenesis.

Indexed as

Aortic Aneurysm, AbdominalExosomesMicroRNAsBiomarkersHumansReal-Time Polymerase Chain ReactionBiomarkersMicroRNAsMIRN122 microRNA, human

Identifiers

PMID36787323
PMCPMC9928131
OpenAlexW4320710409

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.