ArticleJournal of experimental botany2023
The transcription factor POPEYE negatively regulates the expression of bHLH Ib genes to maintain iron homeostasis.
Article in Journal of experimental botany, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 27 citations in OpenAlex.
- Heterologous expression of DobHLH25 from Dendrobium officinale enhances drought tolerance in Arabidopsis.Functional & integrative genomics · 2026Article
- OsbHLH064, an IVb bHLH Transcription Factor, Regulates Iron Homeostasis and Enhances Grain Fe Accumulation in Rice.Plant biotechnology journal · 2026Article
- Expanding Iron Acquisition in Maize: Root Sector-Specific Responses and Gibberellin Regulation of Ferric and Ferrous Iron Uptake.International journal of molecular sciences · 2026Article
- Iron status modulates cadmium tolerance: mechanistic insights fromFrontiers in plant science · 2026Article
- Age-dependent differential iron deficiency responses of rosette leaves during reproductive stages in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- OsbHLH062 regulates iron homeostasis by inhibiting iron deficiency responses in rice.aBIOTECH · 2025Article
- Shedding light on iron nutrition: exploring intersections of transcription factor cascades in light and iron deficiency signaling.Journal of experimental botany · 2025Review
- Understanding the Crucial Role of Phosphate and Iron Availability in Regulating Root Nodule Symbiosis.Plant & cell physiology · 2024Review
- Investigating the Role of Known Arabidopsis Iron Genes in a Stress Resilient Soybean Line.International journal of molecular sciences · 2024Article
- The small iron-deficiency-induced protein OLIVIA and its relation to the bHLH transcription factor POPEYE.PloS one · 2024Article
- Iron sensing in plants.Frontiers in plant science · 2023Review
- Multilayered regulation of iron homeostasis in Arabidopsis.Frontiers in plant science · 2023Review
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2 authors at 1 institution in 1 country.
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Abstract
Iron (Fe) is an essential trace element for plants. When suffering from Fe deficiency, plants modulate the expression of Fe deficiency-responsive genes to promote Fe uptake. POPEYE (PYE) is a key bHLH (basic helix-loop-helix) transcription factor involved in Fe homeostasis. However, the molecular mechanism of PYE regulating the Fe deficiency response remains elusive in Arabidopsis. We found that the overexpression of PYE attenuates the expression of Fe deficiency-responsive genes. PYE directly represses the transcription of bHLH Ib genes (bHLH38, bHLH39, bHLH100, and bHLH101) by associating with their promoters. Although PYE contains an ethylene response factor-associated amphiphilic repression (EAR) motif, it does not interact with the transcriptional co-repressors TOPLESS/TOPLESS-RELATED (TPL/TPRs). Sub-cellular localization analysis indicated that PYE localizes in both the cytoplasm and nucleus. PYE contains a nuclear export signal (NES) which is required for the cytoplasmic localization of PYE. Mutation of the NES amplifies the repression function of PYE, resulting in down-regulation of Fe deficiency-responsive genes. Co-expression assays indicated that three bHLH IVc members (bHLH104, bHLH105/ILR3, and bHLH115) facilitate the nuclear accumulation of PYE. Conversely, PYE indirectly represses the transcription activation ability of bHLH IVc. Additionally, PYE directly negatively regulates its own transcription. This study provides new insights into the Fe deficiency response signalling pathway and enhances the understanding of PYE functions in Arabidopsis.
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