Evidence map›Paper›PMID 36782048›Full record

ArticleCancer gene therapy2023

Cytoplasmic localization of IRF5 induces Wnt5a/E-cadherin degradation and promotes gastric cancer cells metastasis.

Jun Du, Chongqi Sun, Jiaojing Liu, Xiaoli Wang, Xuyang Zhao, Yueyuan Wang, Yadong Ma, Hui Xie, Chenxiang Qi, Qianwen Wang and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Cancer gene therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Jun Du *Department of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Chongqi Sun *Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China.
Jiaojing LiuAssisted Reproductive Centre, Shandong Provincial Maternal and Child Health Care Hospital, Jinan, P.R. China.
Xiaoli WangDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Xuyang ZhaoDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Yueyuan WangDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Yadong MaDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Hui XieDepartment of Implantology, Changzhou Stomatological Hospital, Changzhou, P.R. China.
Chenxiang QiDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.ORCID 0000-0001-8795-6766
Qianwen WangDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Tianxiang XiaDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Fengwen YeDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China.
Yujie ZhangDepartment of Physiology, Nanjing Medical University, Nanjing, P.R. China. zhangyujie@njmu.edu.cn.ORCID 0000-0001-7417-997X
Nanjing Medical University · CNJiangsu Province Hospital · CNShandong Maternal and Child Health Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

IRF5, a nucleoplasm shuttling protein, is a pivotal transcription factor regulating immune system activity. It's well known that immunosuppression is involved in the development of gastric cancer. However, no data exist for the expression and function of IRF5 in gastric cancer. This study demonstrated that IRF5 was cytoplasm-enriched in gastric cancer cells. IRF5 promoted gastric cancer cell migration, which involved the inhibition of Wnt5a and E-cadherin proteins expression. IRF5 (LA) localized in nucleus had no significant effect on Wnt5a and E-cadherin expressions, while mutation of IRF5 (ΔNLS), which prevents IRF5 nuclear translocation, had more impact on these inhibitory effects. In addition, degradation rates of both Wnt5a and E-cadherin were enhanced by resiquimod, an IRF5 agonist. Further in vivo experiments indicated that IRF5 knockout of gastric cancer cells repressed their pulmonary metastasis in nude mice. Finally, the expression and clinical significance of IRF5 were analyzed using gastric cancer tissue microarrays, which suggested that the expression of IRF5 varied procedurally in different progressive stages of gastric cancer. Our data revealed that IRF5 cytoplasmic localization were associated with Wnt5a and E-cadherin degradation and gastric cancer cell metastasis. Inhibiting IRF5 expression and/or its cytoplasmic localization may provide a novel target for gastric cancer therapy.

Indexed as

Stomach NeoplasmsAnimalsCadherinsCell Line, TumorCell MovementCytoplasmInterferon Regulatory FactorsMiceMice, NudeCadherinsInterferon Regulatory Factors

Identifiers

PMID36782048
OpenAlexW4320489783

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.