ArticleThe Plant cell2023
Ascorbic acid-mediated reactive oxygen species homeostasis modulates the switch from tapetal cell division to cell differentiation in Arabidopsis.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Decoding the landscape of cell-type-specific co-expressed transcription factors in soybean.Nature plants · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Exogenous ascorbic acid enhances drought tolerance in Hypericum perforatum L. by modulating antioxidant defense and osmotic adjustment.Scientific reports · 2026Article
- Tapetum uncommon behavior, orbicule development, and pollenkitt: mini-review, with new data on orbicule simulations.Protoplasma · 2025Review
- The basic helix-loop-helix (bHLH) transcription factor DTT1 is part of a paired key that unlocks the tapetum transition in barley anther development.The Plant cell · 2025Article
- Nucleobase-ascorbate transporter OsNAT9 regulates seed vigor and drought tolerance by modulating ascorbic acid homeostasis in rice.The Plant journal : for cell and molecular biology · 2025Article
- Identity Transitions of Tapetum Phases: Insights into Vesicular Dynamics and in Mortem Support During Pollen Maturation.Plants (Basel, Switzerland) · 2025Review
- Plastid-localized ZmENR1/ZmHAD1 complex ensures maize pollen and anther development through regulating lipid and ROS metabolism.Nature communications · 2024Article
- Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems.Journal of plant research · 2024Article
- The molecular dynamics between reactive oxygen species (ROS), reactive nitrogen species (RNS) and phytohormones in plant's response to biotic stress.Plant cell reports · 2024Review
- Application of Exogenous Ascorbic Acid Enhances Cold Tolerance in Tomato Seedlings through Molecular and Physiological Responses.International journal of molecular sciences · 2024Article
- The characteristic analysis of TaTDF1 reveals its function related to male sterility in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- Temperature and light reverse the fertility of rice P/TGMS line ostms19 via reactive oxygen species homeostasis.Plant biotechnology journal · 2024Article
- Revisiting the role of ascorbate oxidase in plant systems.Journal of experimental botany · 2024Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Nanobiotechnology-mediated regulation of reactive oxygen species homeostasis under heat and drought stress in plants.Frontiers in plant science · 2024Review
- Barley TAPETAL DEVELOPMENT and FUNCTION1 (HvTDF1) gene reveals conserved and unique roles in controlling anther tapetum development in dicot and monocot plants.The New phytologist · 2023Article
- Stop vitamins: Low levels of ascorbic acid regulate the transition from cell proliferation to differentiation in Arabidopsis tapetum.The Plant cell · 2023Article
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8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major antioxidant L-ascorbic acid (AsA) plays important roles in plant growth, development, and stress responses. However, the importance of AsA concentration and the regulation of AsA metabolism in plant reproduction remain unclear. In Arabidopsis (Arabidopsis thaliana) anthers, the tapetum monolayer undergoes cell differentiation to support pollen development. Here, we report that a transcription factor, DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1), inhibits tapetal cell division leading to cell differentiation. We identified SKEWED5-SIMILAR 18 (SKS18) as a downstream target of TDF1. Enzymatic assays showed that SKS18, annotated as a multicopper oxidase-like protein, has ascorbate oxidase activity, leading to AsA oxidation. We also show that VITAMIN C DEFECTIVE1 (VTC1), an AsA biosynthetic enzyme, is negatively controlled by TDF1 to maintain proper AsA contents. Consistently, either knockout of SKS18 or VTC1 overexpression raised AsA concentrations, resulting in extra tapetal cells, while SKS18 overexpression in tdf1 or the vtc1-3 tdf1 double mutant mitigated their defective tapetum. We observed that high AsA concentrations caused lower accumulation of reactive oxygen species (ROS) in tapetal cells. Overexpression of ROS scavenging genes in tapetum restored excess cell divisions. Thus, our findings demonstrate that TDF1-regulated AsA balances cell division and cell differentiation in the tapetum through governing ROS homeostasis.
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