ArticleACS biomaterials science & engineering2023
Expansion Culture of Hair Follicle Stem Cells through Uniform Aggregation in Microwell Array Devices.
Article in ACS biomaterials science & engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Matrigel-embedded 3D culture maintains stemness and enhances hair-inductive capacity of human hair follicle epithelial stem cells.Regenerative therapy · 2026Article
- Hair Follicle Organoids Using Human iPSC-Derived Ectodermal Precursor Cells for Hair Regenerative Medicine.ACS biomaterials science & engineering · 2026Article
- Synergistic effects of mesenchymal stem cell secretome and Wnt10b on hair follicle regeneration in a 3D bioengineering model: a preclinical study.Stem cell research & therapy · 2025Article
- Synergizing biomaterials and cellular molecular pathways: a new frontier in hair follicle generation.Journal of translational medicine · 2025Review
- Materials-based hair follicle engineering: Basic components and recent advances.Materials today. Bio · 2024Review
- Epidermal stem cells: skin surveillance and clinical perspective.Journal of translational medicine · 2024Review
- Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device.ACS biomaterials science & engineering · 2024Article
- High-Throughput Screening of 3-Dimensional Co-culture Hair Follicle Mimetic Tissue with an Enhanced Extracellular Matrix for the Screening of Hair Growth-Promoting Compounds.Biomaterials research · 2024Article
- Biological and mechanical influence of three-dimensional microenvironment formed in microwell on multicellular spheroids composed of heterogeneous hair follicle stem cells.Scientific reports · 2023Article
- iPSC-based approach for human hair follicle regeneration.Frontiers in cell and developmental biology · 2023Review
- Advanced medical treatments for hair loss.Cell transplantationReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hair regeneration using hair follicle stem cells (HFSCs) and dermal papilla cells is a promising approach for the treatment of alopecia. One of the challenges faced in this approach is the quantitative expansion of HFSCs while maintaining their hair induction capacity. In this study, HFSC expansion was achieved through the formation of uniform-diameter cell aggregates that were subsequently encapsulated in Matrigel. We designed a microwell array device, wherein mouse HFSCs were seeded, allowed to form loosely packed aggregates for an hour, and then embedded in Matrigel. Quantitative analysis revealed a 20-fold increase in HFSC number in 2 weeks through this culture device. Gene expression of trichogenic stem cell markers in the device-grown cells showed a significant increase compared with that of typical flat substrate Matrigel suspension culture cells. These microwell array-cultured HFSCs mixed with freshly isolated embryonic mesenchymal cells indicated vigorous hair regeneration on the skin of nude mice. Furthermore, we examined the feasibility of this approach for the expansion of human HFSCs from androgenetic alopecia patients and found that the ratio of CD200
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