Evidence map›Paper›PMID 36778248›Full record

ArticlebioRxiv : the preprint server for biology2023

Programmable RNA detection with CRISPR-Cas12a.

Santosh R Rananaware, Emma K Vesco, Grace M Shoemaker, Swapnil S Anekar, Luke Samuel W Sandoval, Katelyn S Meister, Nicolas C Macaluso, Long T Nguyen, Piyush K Jain

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Santosh R RananawareDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Emma K VescoDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Grace M ShoemakerDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Swapnil S AnekarDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Luke Samuel W SandovalDepartment of Biology, CLAS, University of Florida, Gainesville, Florida, USA.
Katelyn S MeisterDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Nicolas C MacalusoDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Long T NguyenDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
Piyush K JainDepartment of Chemical Engineering, University of Florida, Gainesville, Florida, USA.
University of Florida · USUniversity of Florida Health · US

Funding

Discovery and engineering of CRISPR/Cas systemsR35GM147788 · NIGMS · UNIVERSITY OF FLORIDA · PI Piyush K Jain · 2022 to 2026
$1.8M
Rapid detection of Hepatitis C virus using CRISPR/CasR21AI156321 · NIAID · UNIVERSITY OF FLORIDA · PI JAIN, PIYUSH K · 2021 to 2022
$419k
Rapid point-of-care detection of Hepatitis C viral RNA using multiplexed CRISPR/Cas platformsR21AI168795 · NIAID · UNIVERSITY OF FLORIDA · PI JAIN, PIYUSH K · 2022 to 2023
$419k
NIAID NIH HHS R21 AI156321NIAID NIH HHS R21 AI168795NIGMS NIH HHS R35 GM147788
6 · The paper itself

Abstract

CRISPR is a prominent bioengineering tool and the type V CRISPR-associated protein complex, Cas12a, is widely used in diagnostic platforms due to its innate ability to cleave DNA substrates. Here we demonstrate that Cas12a can also be programmed to directly detect RNA substrates without the need for reverse transcription or strand displacement. We discovered that while the PAM-proximal "seed" region of the crRNA exclusively recognizes DNA for initiating Abstract Figure:

Identifiers

PMID36778248
PMCPMC9915489
OpenAlexW4318567994

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.